| Purification and some properties of hamster liver aldehyde oxidase. | |
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MedLine Citation:
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PMID: 10598038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aldehyde oxidase was purified from hamster liver cytosol by ammonium sulfate fractionation, chromatography on DEAE-cellulose and Phenyl-Toyopearl, and HPLC-gel filtration on TSK-gel G3000SW(XL) column. The purified enzyme was homogeneous by the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Its molecular weight was determined to be 144800 by SDS-PAGE and 288000 by HPLC gel filtration. The isoelectric point was pH 5.1. The apparent Km and Vmax for benzaldehyde and 2-hydroxypyrimidine were 19.0 and 4.4 microM, and 165 and 211 nmol/min/mg protein, respectively. The benzaldehyde oxidase activity was markedly inhibited by menadione and chlorpromazine. The substrate specificity was different from those of the enzymes from other animals. |
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Authors:
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K Sugihara; Y Katsuma; C Tanaka; S Kitamura |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 22 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-01-31 Completed Date: 2000-01-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1246-8 Citation Subset: IM |
Affiliation:
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Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Oxidase Aldehyde Oxidoreductases / isolation & purification* Animals Chromatography, Gel Cricetinae Cytosol / enzymology Electrophoresis, Polyacrylamide Gel Guinea Pigs Kinetics Liver / enzymology* Male Mesocricetus Molecular Weight Proteins / chemistry Rabbits Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Proteins; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.3.1/Aldehyde Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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