Document Detail


Purification and some characteristics of the coagulation factor IX from human plasma.
MedLine Citation:
PMID:  1171684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Non-activated coagulation factor IX was purified approx. 10,000-fold from human plasma. The final product was electrophoretically homogeneous and comprised a tingle polypeptide chain with a molecular weight of about 70,000 and a pI of 4.3-4.45. The N-terminal amino acid was glycine. The amino acid and the carbohydrate contents were analysed and a monospecific antiserum to the factor was raised in rabbits.
Authors:
B Osterud; R Flengsrud
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  145     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-11-06     Completed Date:  1975-11-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  469-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Carbohydrates / analysis
Factor IX / analysis,  immunology,  isolation & purification*
Glycine / analysis
Humans
Immune Sera
Molecular Weight
Peptide Chain Termination, Translational
Peptides / analysis
Rabbits / immunology
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Immune Sera; 0/Peptides; 56-40-6/Glycine; 9001-28-9/Factor IX
Comments/Corrections

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