Document Detail


Purification and properties of the sulfur oxygenase/reductase from the acidothermophilic archaeon, Acidianus strain S5.
MedLine Citation:
PMID:  12664265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sulfur oxygenase/reductase (SOR) of Acidianus strain S5 was purified and characterized after expressing the SOR gene in a recombinant strain of Escherichia coli. The N-terminal sequence of the purified SOR protein was the same as the deduced amino acid sequence from previously cloned SOR genes. Enzymatic studies indicated that the SOR catalyzed the conversion of elemental sulfur (S(o)) to sulfite, thiosulfate, and sulfide. The optimal pH and temperature were 5.0 and 70 degrees C, respectively. Comparison of this SOR and that of A. ambivalens revealed several differences between these two SORs. The most striking difference is that the SOR of Acidianus S5 had maximal activity at acidic pH. By application of anti-SOR serum and the Western blot technique, it was found that SOR proteins existed in A. brierleyi and in Acidianus S5 cells cultivated with thiosulfate as the sole energy source, indicating that SOR may also play a role in thiosulfate metabolism.
Authors:
Cui-Wei Sun; Zhi-Wei Chen; Zheng-Guo He; Pei-Jin Zhou; Shuang-Jiang Liu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-01-16
Journal Detail:
Title:  Extremophiles : life under extreme conditions     Volume:  7     ISSN:  1431-0651     ISO Abbreviation:  Extremophiles     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-28     Completed Date:  2003-12-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706854     Medline TA:  Extremophiles     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  131-4     Citation Subset:  IM; S    
Affiliation:
Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, P R China.
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MeSH Terms
Descriptor/Qualifier:
Acidianus / classification,  enzymology*,  genetics
Amino Acid Sequence
Archaeal Proteins*
Escherichia coli / genetics
Genes, Archaeal
Hydrogen-Ion Concentration
Oxidoreductases / genetics,  isolation & purification*,  metabolism
Oxidoreductases Acting on Sulfur Group Donors
Recombinant Proteins / genetics,  metabolism
Species Specificity
Thiosulfates / metabolism
Chemical
Reg. No./Substance:
0/Archaeal Proteins; 0/Recombinant Proteins; 0/Thiosulfates; EC 1.-/Oxidoreductases; EC 1.8.-/Oxidoreductases Acting on Sulfur Group Donors; EC 1.8.-/soR protein, Acidianus ambivalens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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