| Purification and properties of rubrophilin: a novel brain specific membrane polypeptide. | |
| | |
MedLine Citation:
|
PMID: 3806107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Rubrophilin, a unique brain specific polypeptide, was purified to apparent homogeneity from microsomal fractions of bovine brains. The peptide stains pink with Coomassie Brilliant Blue R-250 (C.I. No. 42660) under specific conditions, has an apparent Mr of 53,000, and is acidic with an apparent pI of 4.9. The purification involves initial solubilization of delipidated microsomes in sodium dodecyl sulfate, followed by ammonium sulfate fractionation, reversed ammonium sulfate gradient elution from diatomaceous earth, gel filtration on polyacrylamide (Biogel P-200), gradient elution chromatography from hydroxylapatite, and reverse-phase chromatography from phenyl-Sepharose. A yield of about 5 mg of rubrophilin was obtained from 9 g of microsomal proteins. Amino acid analysis shows that rubrophilin contains only nine amino acids with residues/mol as follows: alanine (102), glutamic acid (97), lysine (65), proline (55), aspartic acid (48), glycine (44), serine (37), threonine (35), and valine (10). Cysteine, methionine, tryptophan, tyrosine, isoleucine, phenylalanine, histidine, and arginine could not be detected. Relative rubrophilin content of vertebrate brains was as follows: mammals greater than birds greater than reptiles greater than fishes. It is present in mouse retina and human neuroblastoma cell cultures but could not be detected in octopus optic lobe or in cultured C-6 rat glioma cells. |
| | |
Authors:
|
H L Rosenthal; B W Moore |
Related Documents
:
|
2919877 - Purification and characterization of a galactoside-binding lectin from human brain. 1747107 - Large-scale purification and characterization of the major phosphoproteins and mucins o... 6221877 - Isolation and characterization of ichthyosan from tuna vitreous. 569157 - Structure and biochemical composition of desmosomes and tonofilaments isolated from cal... 23211517 - Vitamin b-6 restriction impairs fatty acid synthesis in cultured human hepatoma (hepg2)... 12774887 - Protective role of dlalpha-lipoic acid against adriamycin-induced cardiac lipid peroxid... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of neurochemistry Volume: 48 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1987 Mar |
Date Detail:
|
Created Date: 1987-03-19 Completed Date: 1987-03-19 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 832-9 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis Animals Brain Chemistry* Cattle Cell Line Chickens Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Fishes Glioma / analysis Humans Isoelectric Point Membrane Proteins / isolation & purification* Mice Microsomes / analysis* Molecular Weight Nerve Tissue Proteins / isolation & purification* Neuroblastoma / analysis Rats Reptiles Rosaniline Dyes |
| Grant Support | |
ID/Acronym/Agency:
|
NS05270/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Rosaniline Dyes; 0/rubrophilin; 6104-59-2/Coomassie brilliant blue R |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Uncoupling of cerebral glucose supply and utilization after hexane-2,5-dione intoxication in the rat...
Next Document: Inhibition of norepinephrine transport and reserpine binding by reserpine derivatives.