| Purification and properties of polyol dehydrogenase from Cephalosporium chrysogenus. | |
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MedLine Citation:
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PMID: 1374 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A polyol dehydrogenase of broad specificity was purified 178-fold from extracts of the filamentous fungus Cephalosporium chrysogenum. The enzyme was found to act as an oxido-reductase in two substrate-coenzyme systems: D-sorbitol (or xylitol)-nicotinamide-adenine dinucleotide (NAD) and D-mannitol-nicotinamide adenine dinucleotide phosphate (NADP). The dehydrogenase was composed of five isozymes, which, as a mixture, exhibited these properties: Km to D-sorbitol and D-mannitol, 7.15 to 10(-2) M; PH optimum, 9 to 10; molecular weight, 300,000 subunit weight, 29,000; PI, 5.8 to 7.5. The NADP-linked activity was labile to treatment with heat or ethylenediaminetetraacetic acid. Mixed substrate assays support the hypothesis that both NAD-, and NADP-linked activities are associated with isozymes of a single dehydrogenase. |
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Authors:
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S Birken; M A Pisano |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 125 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1976 Jan |
Date Detail:
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Created Date: 1976-04-01 Completed Date: 1976-04-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 225-32 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acremonium
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enzymology* Alcohol Oxidoreductases* / isolation & purification, metabolism Carbohydrate Metabolism Cell-Free System Edetic Acid / pharmacology Fructose / biosynthesis Hot Temperature Hydrogen-Ion Concentration Isoenzymes* / isolation & purification Magnesium / pharmacology Mannitol / metabolism Molecular Weight NAD / metabolism NADP / metabolism Sorbitol / metabolism Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 30237-26-4/Fructose; 50-70-4/Sorbitol; 53-59-8/NADP; 53-84-9/NAD; 60-00-4/Edetic Acid; 69-65-8/Mannitol; 7439-95-4/Magnesium; EC 1.1.-/Alcohol Oxidoreductases |
| Comments/Corrections | |
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