Document Detail


Purification and properties of pituitary gonadotropic hormone from Indian teleosts: freshwater murrel (Channa punctatus) and carp (Catla catla).
MedLine Citation:
PMID:  2920895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gonadotropic hormone (GtH) from the pituitaries of two widely different Indian teleosts, a freshwater murrel and a carp, was purified by solvent fractionation, Sephadex G-100 gel filtration, affinity chromatography on concanavalin A-Sepharose (Con A-Sepharose), and immunoaffinity chromatography. Elution profile from gel filtration showed three peaks in both cases, peak I and II were clearly separated in murrel but not in carp. Peak II demonstrated strong GtH activity in both murrel and carp but this activity could also be detected in peak I and III of carp. TSH activity was restricted to peak I in murrel and was distributed in all three peaks in carp. Chromatography on Con A-Sepharose was useful in harvesting carp glycoprotein hormones, but gave no advantage in murrel. A pure homogenous GtH from murrel and carp could be obtained by using immunoaffinity chromatography. Polyacrylamide gel electrophoresis of murrel and carp GtH showed a single discrete band. Determination of molecular weight (MW) by Sephadex G-100 gel filtration indicated that murrel and carp GtH were 42,000 and 40,000 Da, respectively. SDS-polyacrylamide gel electrophoresis of murrel and carp GtH revealed two dissimilar subunits, alpha and beta. MW of murrel and carp alpha-subunits were 18,000 and 16,000 while those of beta were 27,000 and 26,000 Da, respectively. Comparison of electrophoretic patterns of murrel and carp GtH alpha and beta with ovine LH subunits reveal distinct teleostean GtH subunits.
Authors:
P P Banerjee; S Bhattacharya; P Nath
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  73     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-18     Completed Date:  1989-04-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  118-28     Citation Subset:  IM    
Affiliation:
Department of Zoology, School of Life Science, Visva-Bharati University, West Bengal, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carps / metabolism*
Chromatography, Affinity
Chromatography, Gel
Cyprinidae / metabolism*
Electrophoresis, Polyacrylamide Gel
Fishes / metabolism*
Gonadotropins, Pituitary / isolation & purification*
Sodium Dodecyl Sulfate
Chemical
Reg. No./Substance:
0/Gonadotropins, Pituitary; 151-21-3/Sodium Dodecyl Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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