Document Detail


Purification and properties of a cell-bound bacteriocin from a Bacteroides fragilis strain.
MedLine Citation:
PMID:  6201135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cell-bound bacteriocin was extracted from a Bacteroides fragilis BF-11 strain by treating the cells with a low-molarity buffer (0.01 M Tris-hydrochloride, pH 8.0). Sucrose osmotic shock experiments and ultrasonic lysis of whole cells indicated that the majority of the bacteriocin was located at the cell surface. Culture supernatants contained no significant bacteriocin activity. The bacteriocin was purified by DEAE-cellulose and Sephacryl S200 chromatography and had an apparent molecular weight of approximately 7,000. It was relatively heat stable and was inactivated by proteases. There was a delay of approximately 3.5 h before DNA, RNA, and protein synthesis were inhibited by the bacteriocin. Inhibition of macromolecular synthesis coincided with lysis of the susceptible indicator strain.
Authors:
J A Southern; W Katz; D R Woods
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  25     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-05-04     Completed Date:  1984-05-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  253-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis
Bacteriocins / isolation & purification*
Bacteroides fragilis / analysis*,  ultrastructure
Chemical Phenomena
Chemistry
DNA, Bacterial / biosynthesis
DNA-Directed RNA Polymerases / metabolism
Molecular Weight
RNA, Bacterial / biosynthesis
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacteriocins; 0/DNA, Bacterial; 0/RNA, Bacterial; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

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