| Purification and properties of a cell-bound bacteriocin from a Bacteroides fragilis strain. | |
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MedLine Citation:
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PMID: 6201135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A cell-bound bacteriocin was extracted from a Bacteroides fragilis BF-11 strain by treating the cells with a low-molarity buffer (0.01 M Tris-hydrochloride, pH 8.0). Sucrose osmotic shock experiments and ultrasonic lysis of whole cells indicated that the majority of the bacteriocin was located at the cell surface. Culture supernatants contained no significant bacteriocin activity. The bacteriocin was purified by DEAE-cellulose and Sephacryl S200 chromatography and had an apparent molecular weight of approximately 7,000. It was relatively heat stable and was inactivated by proteases. There was a delay of approximately 3.5 h before DNA, RNA, and protein synthesis were inhibited by the bacteriocin. Inhibition of macromolecular synthesis coincided with lysis of the susceptible indicator strain. |
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Authors:
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J A Southern; W Katz; D R Woods |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Antimicrobial agents and chemotherapy Volume: 25 ISSN: 0066-4804 ISO Abbreviation: Antimicrob. Agents Chemother. Publication Date: 1984 Feb |
Date Detail:
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Created Date: 1984-05-04 Completed Date: 1984-05-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0315061 Medline TA: Antimicrob Agents Chemother Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 253-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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biosynthesis Bacteriocins / isolation & purification* Bacteroides fragilis / analysis*, ultrastructure Chemical Phenomena Chemistry DNA, Bacterial / biosynthesis DNA-Directed RNA Polymerases / metabolism Molecular Weight RNA, Bacterial / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Bacteriocins; 0/DNA, Bacterial; 0/RNA, Bacterial; EC 2.7.7.6/DNA-Directed RNA Polymerases |
| Comments/Corrections | |
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