Document Detail


Purification and properties of an aryl acylamidase of Bacillus sphaericus, catalyzing the hydrolysis of various phenylamide herbicides and fungicides.
MedLine Citation:
PMID:  4762392     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
From Bacillus sphaericus ATCC 12123 an aryl acylamidase (EC 3.5.1.13) was purified to homogeneity by ion exchange chromatography, gel filtration, and polyacrylamide gel electrophoresis. The enzyme is inducible by various phenylamides of the acylanilide, phenylcarbamate, and methoxysubstituted phenylurea type. It has a molecular weight of 75,000. Enzyme activity was inhibited by sulfhydryl reagents, several metal ions, and 3,4-dichloroaniline (a product of linuron degradation). A requirement for divalent metal ions in enzyme activity could not be demonstrated. In the presence of 6 M urea an irreversible inactivation of the enzyme occurred. The hydrolysis of L-alanine-4-nitroanilide was competitively inhibited by puromycin.
Authors:
G Engelhardt; P R Wallnöfer; R Plapp
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  26     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1973 Nov 
Date Detail:
Created Date:  1974-02-12     Completed Date:  1974-02-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  709-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amides / metabolism*
Amidohydrolases / analysis*,  antagonists & inhibitors,  isolation & purification
Bacillus / drug effects,  enzymology*
Buffers
Calcium Phosphates / diagnostic use
Carbon Radioisotopes
Chromatography, DEAE-Cellulose
Chromatography, Gel
Culture Media
Electrophoresis, Polyacrylamide Gel
Fungicides, Industrial*
Herbicides*
Hydrogen-Ion Concentration
Hydrolysis
Kinetics
Linuron / metabolism
Metals / pharmacology
Molecular Weight
Penicillin G / pharmacology
Protamines / diagnostic use
Puromycin / pharmacology
Temperature
Chemical
Reg. No./Substance:
0/Amides; 0/Buffers; 0/Calcium Phosphates; 0/Carbon Radioisotopes; 0/Culture Media; 0/Fungicides, Industrial; 0/Herbicides; 0/Metals; 0/Protamines; 330-55-2/Linuron; 53-79-2/Puromycin; 61-33-6/Penicillin G; EC 3.5.-/Amidohydrolases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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