Document Detail


Purification and partial sequence analysis of human interleukin-2 derived from peripheral blood leukocytes.
MedLine Citation:
PMID:  3872125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH2-terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes.
Authors:
K Kato; K Naruo; M Koyama; K Kawahara; S Hinuma; H Tada; H Sugino; K Tsukamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  127     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-04-15     Completed Date:  1985-04-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  182-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Chromatography, DEAE-Cellulose
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Humans
Interleukin-2 / isolation & purification*
Leukocytes / analysis*
Molecular Weight
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Interleukin-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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