Document Detail

Purification and partial characterization of a procaryotic glycoprotein from the plasma membrane of Thermoplasma acidophilum.
MedLine Citation:
PMID:  534627     Owner:  NLM     Status:  MEDLINE    
The obligate, thermophilic, acidophilic mycoplasma, Thermoplasma acidophilum, grows optimally at 56 degrees C and pH 2.0. Its plasma membrane possessed 21--22 protein bands that were resolved by polyacrylamide gel electrophoresis. One major membrane protein, molecular weight 152 000, which stained for carbohydrate with periodic acid-Schiff reagent, accounted for 32% (w/w) of the total membrane proteins. It was isolated and further purified by concanavalin A affinity chromatography. The carbohydrate content amounted to less than 10% (w/w) compared to that of the entire glycoprotein. The carbohydrate moiety consisted mainly of mannose residues with branched alpha 1 leads to 2 linkages at the non-reducing ends of the glycopeptide as determined by permethylation followed by gas chromatography-mass spectrometry analysis. The reducing end was an N-glycosidic linkage between asparagine and N-acetylglucosamine. The amino acid composition of this glycoprotein showed 62 mol% hydrophobic residues, while the acidic amino acid content contributed 9 mol% more than that of the basic amino acids. The existence of membrane glycoproteins in the procaryotic, wall-less T. acidophilum may provide a protective coat for the plasma membrane. The stereochemistry and the conformation of the carbohydrate chains, in conjunciton with water turgor, may contribute to the rigidity of the membrane and the cation binding.
L L Yang; A Haug
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  556     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Sep 
Date Detail:
Created Date:  1980-05-23     Completed Date:  1980-05-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  265-77     Citation Subset:  IM    
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MeSH Terms
Adaptation, Biological
Amino Acids / analysis
Bacterial Proteins / isolation & purification*
Carbohydrates / analysis
Cell Membrane / analysis
Chemical Phenomena
Glycoproteins / isolation & purification*
Membrane Proteins / isolation & purification*
Thermoplasma / analysis*
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Carbohydrates; 0/Glycoproteins; 0/Membrane Proteins

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