| Purification of oligouronides using hollow-fiber membrane functionalised with L-histidine. | |
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MedLine Citation:
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PMID: 16330277 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pseudobioaffinity chromatography with L-histidines immobilized onto poly(ethylene-vinyl alcohol) hollow-fiber membranes was studied for a selective purification of anionic oligosaccharides. Oligoglucuronans with different degrees of polymerization and acetylation, prepared by an enzymatic degradation of bacterial polyglucuronic acid, were used as models. The adsorption and elution parameters were studied to optimise the selective adsorption. A better understanding of the physico-chemical phenomena governing this selectivity was attempted and a few hypotheses on the mechanism of selectivity are proposed. |
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Authors:
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C Delattre; P Michaud; K Hamze; B Courtois; J Courtois; M A Vijayalakshmi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-21 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1099 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-06 Completed Date: 2006-03-07 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 121-6 Citation Subset: IM |
Affiliation:
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Laboratoire des Glucides-EPMV FRE CNRS 2779, IUT/GB, UPJV, Avenue des Facult?s, Le Bailly, 80025 Amiens Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Biopolymers / isolation & purification* Buffers Histidine / chemistry* Magnetic Resonance Spectroscopy Sinorhizobium meliloti / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Biopolymers; 0/Buffers; 71-00-1/Histidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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