| Purification of normal lymphocytes from leukemic T-cells by lectin-affinity adsorbents - correlation with lectin-cell binding. | |
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MedLine Citation:
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PMID: 12637153 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Utilization of leukemic T-cells from normal ones, using lectin-affinity adsorbents, is described. CNBr-activated Sepharose 6MB was covalently coupled to Soybean (SBA) or Dolichos Biflorus Agglutinins (DBA), then serves as an affinity probe for separation of leukemic T-cells from normal lymphocytes. The normal lymphocytes were removed almost completely by phosphate buffered saline (Ca(2+) and Mg(2+) free) (PBS(-)) from lectin-affinity column. More than 80% of the leukemic T-cells were retained on the lectin-affinity adsorbent, whereas another 10-15% were easily removed by PBS(-). There was a very good linear correlation between percent of cells, retained on the lectin-affinity adsorbent and percent of cells, interacting with the respective free lectin (r=0.97 for SBA, and r=0.93 for DBA). The viability of normal lymphocytes was not influenced after passing through the columns. In the case of leukemic T-cells - about 90% of the easily removed cells were dead, and another 10% were viable cells, non-interacting with DBA or SBA. |
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Authors:
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R Bakalova; H Ohba |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer letters Volume: 192 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-14 Completed Date: 2003-05-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 59-65 Citation Subset: IM |
Affiliation:
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Institute for Structural and Engineering Materials, Independent Administrative Institution, National Institute of Advanced Industrial Science and Technology, AIST-Kyushu, Saga-ken, Japan. r.bakalova-zheleva@aist.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Separation
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methods* Cell Survival Chromatography, Affinity / methods* Flow Cytometry Humans Leukemia / immunology*, pathology* Plant Lectins / metabolism* Soybean Proteins / metabolism* T-Lymphocytes / cytology*, immunology, metabolism*, pathology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Plant Lectins; 0/Soybean Proteins; 0/dolichos biflorus agglutinin; 0/soybean lectin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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