Document Detail


Purification of normal lymphocytes from leukemic T-cells by lectin-affinity adsorbents - correlation with lectin-cell binding.
MedLine Citation:
PMID:  12637153     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Utilization of leukemic T-cells from normal ones, using lectin-affinity adsorbents, is described. CNBr-activated Sepharose 6MB was covalently coupled to Soybean (SBA) or Dolichos Biflorus Agglutinins (DBA), then serves as an affinity probe for separation of leukemic T-cells from normal lymphocytes. The normal lymphocytes were removed almost completely by phosphate buffered saline (Ca(2+) and Mg(2+) free) (PBS(-)) from lectin-affinity column. More than 80% of the leukemic T-cells were retained on the lectin-affinity adsorbent, whereas another 10-15% were easily removed by PBS(-). There was a very good linear correlation between percent of cells, retained on the lectin-affinity adsorbent and percent of cells, interacting with the respective free lectin (r=0.97 for SBA, and r=0.93 for DBA). The viability of normal lymphocytes was not influenced after passing through the columns. In the case of leukemic T-cells - about 90% of the easily removed cells were dead, and another 10% were viable cells, non-interacting with DBA or SBA.
Authors:
R Bakalova; H Ohba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  192     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-14     Completed Date:  2003-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Affiliation:
Institute for Structural and Engineering Materials, Independent Administrative Institution, National Institute of Advanced Industrial Science and Technology, AIST-Kyushu, Saga-ken, Japan. r.bakalova-zheleva@aist.go.jp
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / methods*
Cell Survival
Chromatography, Affinity / methods*
Flow Cytometry
Humans
Leukemia / immunology*,  pathology*
Plant Lectins / metabolism*
Soybean Proteins / metabolism*
T-Lymphocytes / cytology*,  immunology,  metabolism*,  pathology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Plant Lectins; 0/Soybean Proteins; 0/dolichos biflorus agglutinin; 0/soybean lectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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