Document Detail


Purification of murine IgG monoclonal antibodies by precipitation with caprylic acid: comparison with other methods of purification.
MedLine Citation:
PMID:  2784406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Purification of murine IgG monoclonal antibodies from ascitic fluid by precipitation with caprylic acid was compared to that: 1) by sequential precipitation with caprylic acid and (NH4)2SO4; 2) by affinity chromatography on either antiidiotypic monoclonal antibodies or antimouse IgG xenoantibodies; and 3) by sequential (NH4)2SO4 precipitation, ion exchange chromatography and high pressure liquid chromatography (referred to as HPLC). In terms of yield of antibodies, precipitation with caprylic acid is comparable to sequential precipitation with caprylic acid and (NH4)2SO4, but superior to affinity chromatography on antibodies and to HPLC. In terms of purity of antibodies, precipitation with caprylic acid is less efficient than the other three methods. It should also be noted that precipitation with caprylic acid is associated with a reduction in the affinity of some antibodies and is not suitable to purify murine IgA and IgG3.
Authors:
M Temponi; T Kageshita; F Perosa; R Ono; H Okada; S Ferrone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hybridoma     Volume:  8     ISSN:  0272-457X     ISO Abbreviation:  Hybridoma     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-05-05     Completed Date:  1989-05-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8202424     Medline TA:  Hybridoma     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  85-95     Citation Subset:  IM    
Affiliation:
Institute of Chemistry, Faculty of Medicine, University of Brescia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Anti-Idiotypic / isolation & purification
Antibodies, Monoclonal / isolation & purification*
Antibodies, Neoplasm / isolation & purification
Antibody Affinity
Chemical Precipitation
HLA Antigens / immunology
Humans
Immunoglobulin G / isolation & purification*
Immunoglobulin Idiotypes / immunology
Melanoma / immunology
Mice
Octanoic Acids
Grant Support
ID/Acronym/Agency:
AI21384/AI/NIAID NIH HHS; CA37959/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Antibodies, Monoclonal; 0/Antibodies, Neoplasm; 0/HLA Antigens; 0/Immunoglobulin G; 0/Immunoglobulin Idiotypes; 0/Octanoic Acids; 124-07-2/caprylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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