Document Detail


Purification and macromolecular properties of a sialic acid-specific lectin from the slug Limax flavus.
MedLine Citation:
PMID:  7085639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A lectin (LFA) which is highly specific for sialic acid has been purified from the slug Limax flavus by a combination of ammonium sulfate fractionation and affinity chromatography on bovine submaxillary mucin coupled to Sepharose 4B. The affinity-purified lectin appeared homogeneous by electrophoresis in the presence of sodium dodecyl sulfate. Below 1 mg/ml at pH 7, LFA exists as a species of Mr = 44,000 which is composed of two equal sized subunits. Above 1 mg/ml, the protein solution was observed to behave as a rapidly associating-dissociating system. N-acetylneuraminic acid and N-glycolylneuraminic acid gave a 50% inhibition of agglutination of erythrocytes by LFA at 0.13 and 0.81 mM, respectively. Galactose, N-acetylgalactosamine, galactosamine, glucose, N-acetylglucosamine, glucosamine, mannose, arabinose, xylose, fucose, glucuronic acid, alpha-methyl-D-glucoside, alpha-methyl-D-mannoside, lactose, and sucrose were ineffective inhibitors at concentrations up to 10-25 mM. Bovine submaxillary mucin, a sialoprotein, was a potent inhibitor of hemagglutination by LFA. Upon treatment of the mucin with neuraminidase, loss of inhibitory activity was observed which was proportional to the loss of sialic acid from the mucin.
Authors:
R L Miller; J F Collawn; W W Fish
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-08-26     Completed Date:  1982-08-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7574-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Carbohydrates
Cattle
Chromatography, Affinity
Circular Dichroism
Hemagglutination
Lectins / isolation & purification*
Macromolecular Substances
Molecular Weight
Mollusca / analysis*
Mucins
Protein Conformation
Sialic Acids
Spectrophotometry, Ultraviolet
Submandibular Gland
Grant Support
ID/Acronym/Agency:
HL19491/HL/NHLBI NIH HHS; HL26445/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Lectins; 0/Macromolecular Substances; 0/Mucins; 0/Sialic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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