| Purification of lipoxygenase and hydroperoxide dehydrase in flaxseeds: interaction between these enzymatic activities. | |
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MedLine Citation:
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PMID: 1359887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have purified two enzymic activities from flaxseed acetone powder: a lipoxygenase and a hydroperoxide dehydrase. The lipoxygenase activity belongs to an iron-containing protein having a molecular weight of 130 kDa which, upon incubation with alpha-linolenic acid, forms 13-hydroperoxy-9(Z), 11(E), 15(Z)- octadecatrienoic acid. The hydroperoxide dehydrase (a 55 kDa protein) metabolizes this hydroperoxide to an allene oxide which in turn is spontaneously hydrolyzed to alpha- and gamma-ketols. Relationships between these two enzymes were studied and results suggest an inhibition of the lipoxygenase by hydroperoxide dehydrase. |
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Authors:
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H Rabinovitch-Chable; J Cook-Moreau; J C Breton; M Rigaud |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 188 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-12-04 Completed Date: 1992-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 858-64 Citation Subset: IM |
Affiliation:
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U.R.A. C.N.R.S. 1485, Faculté de Médecine, Limoges, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gel Hydro-Lyases / isolation & purification*, metabolism* Kinetics Linolenic Acids / metabolism Lipoxygenase / isolation & purification*, metabolism* Seeds / enzymology* alpha-Linolenic Acid |
| Chemical | |
Reg. No./Substance:
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0/Linolenic Acids; 463-40-1/alpha-Linolenic Acid; EC 1.13.11.12/Lipoxygenase; EC 4.2.1.-/Hydro-Lyases; EC 4.2.1.-/hydroperoxide dehydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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