Document Detail

Purification of and kinetic studies on a narrow specificity synaptosomal membrane pyroglutamate aminopeptidase from guinea-pig brain.
MedLine Citation:
PMID:  2862039     Owner:  NLM     Status:  MEDLINE    
A pyroglutamate aminopeptidase activity, distinct from that of cytoplasm, was released from a synaptosomal membrane preparation of guinea-pig brain by papain treatment. This activity was further purified 3560-fold relative to the homogenate with a yield of 17% by a procedure involving gel filtration chromatography, calcium phosphate cellulose chromatography and hydrophobic interaction chromatography on phenyl-Sepharose CL-4B. The purified synaptosomal pyroglutamate aminopeptidase hydrolysed only thyroliberin, acid-thyroliberin, the luliberin N-terminal tripeptide (Glp-His-Trp) and, only slightly, Glp-His-Gly. No hydrolysis was observed with dipeptides containing N-terminal pyroglutamic acid (Glp) or with pyroglutamyl peptides containing more than three amino acids. A Km value of 40 microM was recorded when thyroliberin was used as substrate; however, luliberin was found to inhibit the hydrolysis of thyroliberin competitively with a Ki value of 20 microM.
B O'Connor; G O'Cuinn
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  150     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1985 Jul 
Date Detail:
Created Date:  1985-08-28     Completed Date:  1985-08-28     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  47-52     Citation Subset:  IM    
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MeSH Terms
Aminopeptidases / isolation & purification*
Binding, Competitive
Brain / enzymology*
Chromatography / methods
Electrophoresis / methods
Guinea Pigs
Peptides / metabolism,  pharmacology
Pyroglutamyl-Peptidase I / antagonists & inhibitors,  isolation & purification*,  metabolism
Substrate Specificity
Synaptosomes / enzymology*
Thyrotropin-Releasing Hormone / metabolism
Reg. No./Substance:
0/Peptides; 24305-27-9/Thyrotropin-Releasing Hormone; EC 3.4.11.-/Aminopeptidases; EC I

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