Document Detail


Purification and isolation of corticosteroidogenic cells from head kidney of rainbow trout (Oncorhynchus mykiss) for testing cell-specific effects of a pesticide.
MedLine Citation:
PMID:  17719278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The teleost head kidneys contain corticosteroidogenic cells, chromaffin cells, lymphoid cells, and melanomacrophages. We have developed and validated a method using a Percoll density gradient and differential staining for 3beta-hydroxysteroid dehydrogenase (3beta-HSD) to prepare fractions enriched with specific head kidney cell types. The proportion of steroidogenic cells to other cells in the head kidney was 1:8000 in rainbow trout, Oncorhynchus mykiss. To test the hypothesis that steroidogenic cells are more vulnerable to a pesticide than other cell types in the head kidney, head kidney cells were separated by a Percoll gradient and the steroidogenic cell-enriched fractions and lymphoid cell-enriched fractions were exposed to the pesticide endosulfan in vitro, and their functional integrity and viability were assessed. The effective concentration of the pesticide (EC50, concentration that inhibits 50% of the secretory response to ACTH) in the mixed head kidney cell preparation was similar to the EC50 in the fraction enriched with steroidogenic cells, but differences in viability were detected. The Percoll method for isolation of different cell types from the head kidney facilitated a study of cell-specific effects of a pesticide.
Authors:
Alice Hontela; Vincent S Leblond; John P Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2007-08-01
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  147     ISSN:  1532-0456     ISO Abbreviation:  Comp. Biochem. Physiol. C Toxicol. Pharmacol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-14     Completed Date:  2008-04-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  52-60     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada T1K 3M4. alice.hontela@uleth.ca
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxysteroid Dehydrogenases / analysis
Adrenal Cortex Hormones / biosynthesis*
Adrenocorticotropic Hormone / metabolism
Animals
Cell Separation / methods*
Cell Shape
Cell Survival / drug effects
Centrifugation, Density Gradient*
Dose-Response Relationship, Drug
Endosulfan / toxicity*
Fish Proteins / analysis
Hydrocortisone / metabolism
Kidney / drug effects*,  enzymology,  metabolism,  pathology
Lymphocytes / drug effects*,  enzymology,  metabolism,  pathology
Oncorhynchus mykiss / metabolism*
Pesticides / toxicity*
Povidone
Reproducibility of Results
Silicon Dioxide
Staining and Labeling
Chemical
Reg. No./Substance:
0/Adrenal Cortex Hormones; 0/Fish Proteins; 0/Pesticides; 115-29-7/Endosulfan; 50-23-7/Hydrocortisone; 65455-52-9/Percoll; 7631-86-9/Silicon Dioxide; 9002-60-2/Adrenocorticotropic Hormone; 9003-39-8/Povidone; EC 1.1.-/3-Hydroxysteroid Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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