Document Detail

Purification of isoacceptor transfer RNA by affinity coupling to aldehyde-containing matrix.
MedLine Citation:
PMID:  6193736     Owner:  NLM     Status:  MEDLINE    
A method has been developed for the affinity coupling of aminoacyl-tRNA to an aldehyde-containing polymer by means of reduction with sodium cyanoborohydride. Sephadex dialdehyde obtained from periodate oxidation of Sephadex G-50, and Enzacryl polyaldehyde obtained from hydrolysis of Enzacryl polyacetal, were found to provide the requisite polymeric matrix. This coupling reaction permitted the rapid purification of isoacceptor tRNAs of Escherichia coli for the alpha-amino acids glycine, valine, and arginine, as well as the alpha-imino acid proline.
Q S Wang; J T Wong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  131     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-10-21     Completed Date:  1983-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  360-4     Citation Subset:  IM    
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MeSH Terms
Amino Acids / genetics*
Arginine / genetics
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Escherichia coli / genetics*
Glycine / genetics
Proline / genetics
RNA, Bacterial / isolation & purification*
RNA, Transfer / isolation & purification*
Valine / genetics
Reg. No./Substance:
0/Amino Acids; 0/RNA, Bacterial; 147-85-3/Proline; 56-40-6/Glycine; 7004-03-7/Valine; 74-79-3/Arginine; 9014-25-9/RNA, Transfer

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