Document Detail

Purification of immunolobulin G Fc-reactive factor from Streptococcus azgazardah.
MedLine Citation:
PMID:  793296     Owner:  NLM     Status:  MEDLINE    
A factor from Streptococcus azgazardah (Lancefield's group C) capable of reacting with the Fc fragment of human IgG was partially purified and characterized. The bacteria were lysed by infection with phage, and the IgG Fc-reactive factor was purified from the crude lysate by affinity chromatography on a IgG-coupled Sepharose 4B column. The column was eluted with 3 M KSCN and the eluate gel-filtered on a Sephadex G 75 column. A 30-fold purification was obtained. The molecular weight of the IgG Fc-reactive factor was estimated to be 60,000. The factor was sensitive to trypsin treatment and to heating 95 degrees C, 10 min at pH 2.0. On polyacrylamide electrophoresis only one band was obtained. The trypsin-and heat-sensitivity, and the estimated molecular weight of the factor was similar to an IgG Fc-reactive factor preparation obtained from group A, type M 56 streptococci by heat treatment at 120 degrees C for 30 min.
P Christensen; S E Holm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta pathologica et microbiologica Scandinavica. Section C, Immunology     Volume:  84     ISSN:  0304-1328     ISO Abbreviation:  Acta Pathol Microbiol Scand C     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1977-01-29     Completed Date:  1977-01-29     Revised Date:  2009-06-04    
Medline Journal Info:
Nlm Unique ID:  7508469     Medline TA:  Acta Pathol Microbiol Scand C     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  196-202     Citation Subset:  IM    
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MeSH Terms
Chromatography, Affinity
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Hot Temperature
Immunoglobulin Fc Fragments* / analysis
Molecular Weight
Streptococcus / immunology*
Streptococcus pyogenes / immunology
Trypsin / pharmacology
Reg. No./Substance:
0/Immunoglobulin Fc Fragments; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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