Document Detail


Purification of immunolobulin G Fc-reactive factor from Streptococcus azgazardah.
MedLine Citation:
PMID:  793296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A factor from Streptococcus azgazardah (Lancefield's group C) capable of reacting with the Fc fragment of human IgG was partially purified and characterized. The bacteria were lysed by infection with phage, and the IgG Fc-reactive factor was purified from the crude lysate by affinity chromatography on a IgG-coupled Sepharose 4B column. The column was eluted with 3 M KSCN and the eluate gel-filtered on a Sephadex G 75 column. A 30-fold purification was obtained. The molecular weight of the IgG Fc-reactive factor was estimated to be 60,000. The factor was sensitive to trypsin treatment and to heating 95 degrees C, 10 min at pH 2.0. On polyacrylamide electrophoresis only one band was obtained. The trypsin-and heat-sensitivity, and the estimated molecular weight of the factor was similar to an IgG Fc-reactive factor preparation obtained from group A, type M 56 streptococci by heat treatment at 120 degrees C for 30 min.
Authors:
P Christensen; S E Holm
Related Documents :
16114256 - Preparation and testing of stationary phases and modified capillaries for affinity chro...
20920456 - A high-affinity recombinant antibody permits rapid and sensitive direct detection of my...
24628386 - Airborne trifluoroacetic acid and its fraction from the degradation of hfc-134a in beij...
7496486 - Purification of monoclonal antibodies on dextran-coated silica support grafted by thiop...
18362036 - Oxidation of elemental mercury using atmospheric pressure non-thermal plasma.
2520236 - Ion-spray mass spectrometry of marine neurotoxins.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta pathologica et microbiologica Scandinavica. Section C, Immunology     Volume:  84     ISSN:  0304-1328     ISO Abbreviation:  Acta Pathol Microbiol Scand C     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1977-01-29     Completed Date:  1977-01-29     Revised Date:  2009-06-04    
Medline Journal Info:
Nlm Unique ID:  7508469     Medline TA:  Acta Pathol Microbiol Scand C     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  196-202     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, Affinity
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Hot Temperature
Immunoglobulin Fc Fragments* / analysis
Molecular Weight
Streptococcus / immunology*
Streptococcus pyogenes / immunology
Trypsin / pharmacology
Chemical
Reg. No./Substance:
0/Immunoglobulin Fc Fragments; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The effect of anti-lymphocyte serum, mitogens and enzymatic treatment on the agglutination and surfa...
Next Document:  Visualization of the binding of IgG myeloma proteins to some group A streptococci.