Document Detail


Purification and identification of linoleic acid hydroperoxides generated by soybean seed lipoxygenases 2 and 3.
MedLine Citation:
PMID:  15998134     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been known that lipoxygenase (LOX) isozymes exhibit differences in product formation, but most product information to date is for LOX 1 among soybean (Glycine max) LOX isozymes. In this study, LOXs 2 and 3 were purified and used to generate hydroperoxide (HPOD) products in an in vitro system using linoleic acid as a substrate in the presence of either air or O2. The products were analyzed to determine their stereoisomeric characteristics. The control (no enzyme) showed only low levels of hydroperoxide production and no stereoisomeric specificity. LOX 2 shows high specificity in product formation, producing roughly 4 times more 13-HPOD than 9-HPOD, nearly all of which was 13-S(Z,E)-HPOD. LOX 3 produced more 9-HPOD than 13-HPOD at approximately a 2:1 ratio. No single stereoisomer was predominant among LOX 3 products. These results demonstrate that different isozymes of LOX have characteristic product profiles in in vitro reactions.
Authors:
Hirotada Fukushige; Cunxi Wang; Thomas D Simpson; Harold W Gardner; David F Hildebrand
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-06     Completed Date:  2005-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5691-4     Citation Subset:  IM    
Affiliation:
Department of Agronomy, University of Kentucky, Lexington, Kentucky 40546, USA.
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MeSH Terms
Descriptor/Qualifier:
Linoleic Acids / analysis*,  metabolism
Lipid Peroxides / analysis*,  metabolism
Lipoxygenase / metabolism*
Seeds / enzymology*
Soybeans / enzymology*
Stereoisomerism
Substrate Specificity
Chemical
Reg. No./Substance:
0/Linoleic Acids; 0/Lipid Peroxides; 25657-09-4/linoleic acid hydroperoxide; EC 1.13.11.-/lipoxygenase 3; EC 1.13.11.12/Lipoxygenase

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