Document Detail


Purification and identification of a human dermal extract component inhibitory to fibroblast proliferation.
MedLine Citation:
PMID:  11448099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It was previously shown that a citric acid buffer extract of human dermis (extract D) inhibited growth of human diploid fibroblasts in monolayer culture (Muir et al., 1997). Further fractionation has shown that the active principle is probably a proteoglycan, and that retention of its inhibitory activity is dependent on the use protease inhibitors throughout the extraction procedure. Elution of extract D from a DEAE-cellulose column produced four major peaks, each of which was subjected to SDS-PAGE as well as being tested for inhibitory activity on the growth of fibroblasts in culture. Peaks III and IV had no inhibitory effect, but peak I contained highly active material. Gels of this peak showed prominent bands of 120 kDa (corresponding to dermatan sulphate proteoglycan II, DS-PG II) and at 45 kDa (corresponding to the core protein). The latter band became more prominent when extract D which had been treated with chrondroitinase ABC was electrophoresed. Their identities were verified by Western blotting. Peak II also contained some slower-acting inhibitory material which has as yet to be identified, but contains little or no protein corresponding to the decorin core-protein. The data indicate that the intact decorin molecule, DS-PG II, is the main inhibitory principle in human skin.
Authors:
J E Stewart; D N Wheatley; J D Holmes; I F Muir
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  25     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2001  
Date Detail:
Created Date:  2001-07-12     Completed Date:  2001-10-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  607-12     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Department of Cell Pathology, University of Aberdeen, MacRobert Building, 581 King Street, Aberdeen AB24 5UA, UK.
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MeSH Terms
Descriptor/Qualifier:
Cell Division / drug effects
Cells, Cultured
Chondroitin ABC Lyase / chemistry
Chromatography, Ion Exchange
Dermis / cytology*
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix Proteins
Fibroblasts / cytology*,  drug effects
Humans
Proteoglycans / isolation & purification,  pharmacology*
Tissue Extracts / pharmacology
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/Proteoglycans; 0/Tissue Extracts; 0/decorin; EC 4.2.2.20/Chondroitin ABC Lyase

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