| Purification and identification of a 7.6-kDa protein in media conditioned by superinvasive cancer cells. | |
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MedLine Citation:
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PMID: 17593624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Selection of the human drug sensitive and invasive cell line (MDA-MB-435S-F) with the chemotherapeutic agent paclitaxel, resulted in the development of drug resistant cell lines displaying enhanced invasion-related characteristics. MATERIALS AND METHODS: Serum-free conditioned media from the human cancer drug-sensitive and invasive cell line (MDA-MB-435S-F) and its paclitaxel-resistant superinvasive variant (MDA-MB-435S-F/Taxol10p4pSI) were analyzed using Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). RESULTS: A differentially expressed protein was observed at 7.6 kDa, which was 4-fold up-regulated in MDA-MB-435S-F/Taxol10p4pSI. The differentially expressed protein was identified using matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS), as a fragment of bovine transferrin. The transferrin receptor was also found to be overexpressed in the superinvasive cell line. CONCLUSION: Cleavage of serum proteins such as transferrin could provide a valuable source of markers for malignant tumours and could also play a role in aspects of cancer pathogenesis, such as tumour cachexia. |
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Authors:
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P Dowling; P Maurya; P Meleady; S A Glynn; A J Dowd; M Henry; M Clynes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 27 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2007 May-Jun |
Date Detail:
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Created Date: 2007-06-27 Completed Date: 2007-07-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 1309-17 Citation Subset: IM |
Affiliation:
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National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. paul.dowling@dcu.ie |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Breast Neoplasms / chemistry*, drug therapy, metabolism, pathology Cell Line, Tumor Culture Media, Conditioned Drug Resistance, Neoplasm Female Humans Neoplasm Invasiveness Neoplasm Proteins / chemistry, isolation & purification* Paclitaxel / pharmacology Peptide Fragments / chemistry, isolation & purification* Receptors, Transferrin / biosynthesis, chemistry, isolation & purification Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods Transferrin / chemistry, isolation & purification* Tumor Markers, Biological / chemistry, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Neoplasm Proteins; 0/Peptide Fragments; 0/Receptors, Transferrin; 0/Tumor Markers, Biological; 11096-37-0/Transferrin; 33069-62-4/Paclitaxel |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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