Document Detail


Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine(®) Sulfate) for application in the process of vaccine production.
MedLine Citation:
PMID:  22530951     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Affinity chromatography using sulfated, spherical cellulose beads (Cellufine(®) Sulfate) was assessed for the purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful to remove chicken egg-derived impurities from allantoic fluids containing influenza viruses, the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that the purification of influenza viruses using Cellufine(®) Sulfate is a practical method as a primary purification in the process of influenza vaccine production.
Authors:
Yoshihiro Sakoda; Masatoshi Okamatsu; Norikazu Isoda; Naoki Yamamoto; Koichi Ozaki; Yasuto Umeda; Shigeyuki Aoyama; Hiroshi Kida
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-24
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Technical Support Team, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Minamata Headquarters, JNC Corporation, 867-8501, Minamata, Kumamoto, Japan Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Resettable Fluorescence Logic Gate Based on Calcein/Layered Double Hydroxide Ultrathin Films.
Next Document:  Adeno-associated virus-mediated doxycycline-regulatable TRAIL expression suppresses growth of human ...