Document Detail

Purification of food-grade oligosaccharides using immobilised cells of Zymomonas mobilis.
MedLine Citation:
PMID:  11935179     Owner:  NLM     Status:  MEDLINE    
Immobilised cells of the bacterium Zymomonas mobilis were used to remove glucose, fructose, and sucrose from food-grade oligosaccharide mixtures. Unpurified fructo-, malto-, isomalto-, gentio-, and inulinoligosaccharides, containing total carbohydrate concentrations of 300 g l(-1), were added to immobilised cells, in 100 ml batch reactors. No pH control or nutrient additions were required. Contaminating glucose, fructose, and sucrose within the mixtures was completely fermented within 12 h. The fermentation end products were ethanol and carbon dioxide. A minor amount of sorbitol was also produced as a fermentation by-product in the inulin-oligosaccharide mixture. No degradation of the oligosaccharides in the mixtures was observed.
R G Crittenden; M J Playne
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-12-20
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  58     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-08     Completed Date:  2002-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  297-302     Citation Subset:  IM    
Food Science Australia, Victoria.
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MeSH Terms
Bacteriological Techniques
Carbon Dioxide / chemical synthesis
Cell Culture Techniques
Chromatography, High Pressure Liquid / methods
Ethanol / chemical synthesis
Food Technology*
Fructose / analysis,  biosynthesis,  metabolism
Glucose / analysis,  biosynthesis,  metabolism
Oligosaccharides / analysis,  isolation & purification*,  metabolism
Sucrose / analysis,  metabolism
Zymomonas / cytology,  metabolism*
Reg. No./Substance:
0/Oligosaccharides; 124-38-9/Carbon Dioxide; 30237-26-4/Fructose; 50-99-7/Glucose; 57-50-1/Sucrose; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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