| Purification of an endogenous 68 kD inhibitor of calcium-activated neutral proteinase (CANP) from bovine brain: immunoblot identification and characterization. | |
| | |
MedLine Citation:
|
PMID: 2319618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A calcium-activated neutral proteinase (CANP)-specific endogenous inhibitor (calpastatin) was purified from bovine brain by successive column chromatography. The purified inhibitor exhibited a major band on sodium dodecylsulfate polyacrylamide gel electrophoresis with an approximate molecular weight of 68 kD. The polyclonal antisera raised to the inhibitor strongly reacted with the 68 kD protein band. Two lightly stained bands approximately 55-68 kD and 120-130 kD were also recognized by the inhibitor antiserum. The inhibitor specifically inhibited CANP activity and the half-maximal inhibition was found with 75 ng of calpastatin per 1 micrograms of CANP in a final volume of 125 microliters. Cathepsin B and papain were not inhibited by the inhibitor, while trypsin and chymotrypsin were inhibited to some extent. The inhibitor formed a complex with CANP and the inactive complex was dissociated into active fractions of enzyme and calpastatin in the presence of EGTA. |
| | |
Authors:
|
N L Banik; A K Chakrabarti; E L Hogan |
Related Documents
:
|
12693978 - Isolation, purification, and properties of cysteine proteinase from bombyx mori l. eggs. 12628378 - Purification and characterization of cathepsin l in arrowtooth flounder (atheresthes st... 3304428 - Purification and properties of a proteinase from vipera lebetina (snake) venom. 3300708 - Partial purification and characterization of a neutral proteinase with collagen telopep... 14048 - Carcinoembryonic character of gamma-glutamyltranspeptidase in primary hepatocellular ca... 16636468 - Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases f... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of neuroscience research Volume: 25 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 1990 Jan |
Date Detail:
|
Created Date: 1990-05-08 Completed Date: 1990-05-08 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 119-24 Citation Subset: IM |
Affiliation:
|
Department of Neurology, Medical University of South Carolina, Charleston. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain / metabolism* Cattle Glycoproteins / isolation & purification* Molecular Weight |
| Grant Support | |
ID/Acronym/Agency:
|
NS-11066/NS/NINDS NIH HHS; NS-21353/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Glycoproteins; 0/calpain inhibitors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mediastinal radiographic abnormality caused by anomalous venous drainage: how often is it seen in Af...
Next Document: Asymmetry of interhemispheric responses evoked in the prefrontal cortex of the rat.