Document Detail


Purification of cytoplasmic antigens from the mycelial phase of Candida albicans: possible advantages of its use in Candida serology.
MedLine Citation:
PMID:  6999358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The serology of candidiasis is complicated by the use of poorly defined antigens. Total extracts of the yeast phase have been commonly used as "cytoplasmic' antigen, without regard to the significant amounts of carbohydrate that may contaminate such preparations. This is particularly true in the case of commercially available antigens that have been used as cytoplasmic antigens but actually are richer in carbohydrate than in protein. Affinity chromatography in concanavalin A - Sepharose provides a simple procedure to separate carbohydrates, mainly mannan, from protein antigens in whole Candida extracts. By using mannan-poor antigens, the specificity of serological reactions can be increased considerably, since both the positive reactions seen in a-symptomatic donors and the cross-reactions seen in patients infected with other fungi are due to anti-mannan antibodies. In contrast, both anti-mannan and anti-cytoplasmic antigen antibodies can be detected in patients suspected of systemic candidiasis. On the other hand, absolute specificity may never be achieved for systemic candidiasis. We have found antibodies against cytoplasmic antigen in a patient allergic to C. albicans, in whom the microorganism was isolated from fecal material. It appears that, under favorable conditions, mucosal sensitization may also trigger as systemic reaction directed against both mannan and cytoplasmic antigens.
Authors:
M Munoz; G Estes; M Kilpatrick; A Di Salvo; G Virella
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mycopathologia     Volume:  72     ISSN:  0301-486X     ISO Abbreviation:  Mycopathologia     Publication Date:  1980 Aug 
Date Detail:
Created Date:  1980-12-16     Completed Date:  1980-12-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7505689     Medline TA:  Mycopathologia     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  47-53     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens, Fungal / analysis,  isolation & purification*
Candida albicans / immunology*
Candidiasis / diagnosis
Carbohydrates / analysis
Precipitin Tests
Serologic Tests
Grant Support
ID/Acronym/Agency:
CA-25746/CA/NCI NIH HHS; HD-09938/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Fungal; 0/Carbohydrates

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