Document Detail

Purification of conjugated linoleic acid isomers through a process including lipase-catalyzed selective esterification.
MedLine Citation:
PMID:  12843679     Owner:  NLM     Status:  MEDLINE    
A mixture of conjugated linoleic acids (CLAs) was prepared by alkali conjugation of high purity linoleic acid. The preparation contained 45.1 wt% cis-9, trans-11 (c9,t11)-CLA, 46.8 wt% trans-10, cis-12 (t10,c12)-CLA, and 5.3 wt% other CLAs. A process comprising Candida rugosa lipase-catalyzed selective esterification with lauryl alcohol, molecular distillation, and urea adduct fractionation under strict conditions in ethanol was very effective for purification of c9,t11- and t10,c12-CLAs. In particular, the urea adduct fractionation efficiently eliminated CLAs except c9,t11- and t10,c12-isomers. Purification of c9,t11- and t10,c12-CLAs from 1.0 kg of the CLA mixture increased the c9,t11-CLA purity to 93.1% with 34% recovery of the initial content, and increased the t10,c12-CLA purity to 95.3% with 31% recovery.
Toshihiro Nagao; Yoshie Yamauchi-Sato; Akio Sugihara; Toshio Iwata; Koji Nagao; Teruyoshi Yanagita; Shuji Adachi; Yuji Shimada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  67     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-04     Completed Date:  2004-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1429-33     Citation Subset:  IM    
Osaka Municipal Technical Research Institute, 1-6-50 Morinomiya, Joto-ku, Osaka 536-8553, Japan.
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MeSH Terms
Candida / enzymology
Chemical Fractionation
Indicators and Reagents
Linoleic Acid / isolation & purification*,  metabolism
Lipase / metabolism*
Reg. No./Substance:
0/Indicators and Reagents; 2197-37-7/Linoleic Acid; EC

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