| Purification and chemical characterization of the major neurotoxin from the venom of Pelamis plautrus. | |
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MedLine Citation:
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PMID: 1148209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A major toxin was isolated from the venom of the sea snake Pelamis platurus (yellow-bellied sea snake) by Sephadex G-50 and carboxymethylcellulose column chromatography. The LD50 of the pure toxin (Pelamis toxin a) was 0.044 mug/g in mice representing a tenfold increase in toxicity after purification. The toxin was homogeneous in acrylamide disc gel electrophoresis and eluted as a single peak after isoelectric focusing in a sucrose density gradient column. The isoelectric point was 9.69; thus it is a highly basic protein. The toxin contained 55 amino acid residues with four disulfide linkages. When all disulfide linkages were reduced and alkylated, the toxic action of the pure toxin disappeared leading to the conclusion that the disulfide bonds of the neurotoxin were essential for toxic action. |
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Authors:
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A T Tu; T S Lin; A L Bieber |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 14 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1975 Jul |
Date Detail:
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Created Date: 1975-10-30 Completed Date: 1975-10-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3408-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Carbohydrates / analysis Chromatography, Gel Chromatography, Ion Exchange Drug Stability Electrophoresis, Disc Isoelectric Focusing Lethal Dose 50 Mice Molecular Weight Seawater Snake Venoms / analysis* Toxins, Biological* / isolation & purification, pharmacology Venoms / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Snake Venoms; 0/Toxins, Biological; 0/Venoms |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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