Document Detail


Purification and characterization of a phospholipase A2 from human ileal mucosa.
MedLine Citation:
PMID:  8399335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We purified a phospholipase A2 (PLA2) from human ileal mucosa to homogeneity. Its NH2-terminal amino acid sequence, amino acid composition, molecular weight, and elution behavior on reverse phase high-performance liquid chromatography were identical to those of human group II PLA2 purified from synovial fluid or spleen. The ileal PLA2 preferred anionic phosphatidylglycerol as substrate. On immunoblot analysis, human ileal mucosa gave more intense immunoreactivity with anti-human synovial fluid PLA2 antibody, at the same position as the purified enzyme, than the cecal mucosa. Northern blot analysis also showed that the level of group II PLA2 mRNA in the ileal mucosa was greater than that in the cecal mucosa. The enzyme was rather uniformly distributed over the colonic mucosa, from cecum to sigmoid colon. These results indicate that the ileal mucosa contains group II PLA2, and that its expression in the ileal mucosa was higher than that in the colonic mucosa.
Authors:
T Minami; H Tojo; Y Shinomura; Y Matsuzawa; M Okamoto
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1170     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-11-15     Completed Date:  1993-11-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  125-30     Citation Subset:  IM    
Affiliation:
Second Department of Internal Medicine, Osaka University Medical School, Suita, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Chromatography, High Pressure Liquid
Colon / enzymology
Crohn Disease / blood,  enzymology
Humans
Ileum / enzymology*
Intestinal Mucosa / enzymology*
Molecular Sequence Data
Molecular Weight
Phosphatidylglycerols / metabolism
Phospholipases A / chemistry,  isolation & purification*
Phospholipases A2
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Phosphatidylglycerols; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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