Document Detail


Purification, characterization, and pathogenicity of urease produced by Vibrio parahaemolyticus.
MedLine Citation:
PMID:  8852357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vibrio parahaemolyticus is a halophilic bacterium associated with gastroenteritis and traveller's diarrhea. The urease-positive, Kanagawa-negative V. parahaemolyticus had been isolated from patients and the environment in the Pacific Northwest, first reported by Kelly et al. (5). Recently, we purified the urease produced by a clinical isolated of V. parahaemolyticus, and its characterization and pathogenicity has been studied. The urease isolation procedure included a water extraction and anion exchange chromatography. The molecular weight for the native enzyme was 275 KDa, and the three subunits of 85 KDa, 59 KDa, and 33 KDa were determined. The isoelectric focusing of urease was 5.2. The purified urease also can cause intestinal fluid accumulation and demonstrate a positive result in the suckling mouse test. These results suggested that the urease produced by V. parahaemolyticus may be another important indicator for the pathogenesis of the bacteria.
Authors:
Y Cai; Y Ni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  10     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-06     Completed Date:  1996-12-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  70-3     Citation Subset:  IM    
Affiliation:
Department of Clinical Laboratory, Shanghai No. 2 Medical University, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Suckling
Electrophoresis, Polyacrylamide Gel
Hot Temperature
Hydrogen-Ion Concentration
Isoelectric Focusing
Mice
Molecular Weight
Rabbits
Urease / chemistry,  isolation & purification*,  metabolism
Vibrio parahaemolyticus / chemistry,  enzymology*,  pathogenicity
Chemical
Reg. No./Substance:
EC 3.5.1.5/Urease

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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