| Purification and characterization of a novel lyase from Cellulomonas sp. that degrades Fusarium and Gibberella acidic polysaccharides. | |
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MedLine Citation:
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PMID: 1368728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-beta-D-mannopyranosyl-(1----2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peaks (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-beta-D-mannopyranosyl-(1----2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures. |
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Authors:
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K Takegawa; S Yamaguchi; S Miki; T Jikibara; S Iwahara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Agricultural and biological chemistry Volume: 55 ISSN: 0002-1369 ISO Abbreviation: Agric. Biol. Chem. Publication Date: 1991 Aug |
Date Detail:
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Created Date: 1992-02-26 Completed Date: 1992-02-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370452 Medline TA: Agric Biol Chem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1969-75 Citation Subset: B |
Affiliation:
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Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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isolation & purification,
metabolism* Carbohydrate Sequence Fusarium / metabolism* Gibberella / metabolism* Gram-Positive Asporogenous Rods / enzymology* Lyases / isolation & purification, metabolism* Molecular Sequence Data Molecular Weight Polysaccharides / metabolism* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Polysaccharides; EC 4.-/Lyases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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