Document Detail


Purification and characterization of a novel lyase from Cellulomonas sp. that degrades Fusarium and Gibberella acidic polysaccharides.
MedLine Citation:
PMID:  1368728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-beta-D-mannopyranosyl-(1----2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peaks (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-beta-D-mannopyranosyl-(1----2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures.
Authors:
K Takegawa; S Yamaguchi; S Miki; T Jikibara; S Iwahara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Agricultural and biological chemistry     Volume:  55     ISSN:  0002-1369     ISO Abbreviation:  Agric. Biol. Chem.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1992-02-26     Completed Date:  1992-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370452     Medline TA:  Agric Biol Chem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1969-75     Citation Subset:  B    
Affiliation:
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / isolation & purification,  metabolism*
Carbohydrate Sequence
Fusarium / metabolism*
Gibberella / metabolism*
Gram-Positive Asporogenous Rods / enzymology*
Lyases / isolation & purification,  metabolism*
Molecular Sequence Data
Molecular Weight
Polysaccharides / metabolism*
Substrate Specificity
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Polysaccharides; EC 4.-/Lyases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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