| Purification and characterization of a novel glutathione S-transferase from Asaphis dichotoma. | |
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MedLine Citation:
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PMID: 12139969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An isoenzyme of glutathione S-transferase (adGST) was purified from liver intestine of the seashell (Asaphis dichotoma) by GST-Sepharose 4B affinity chromatography followed by reverse-phase HPLC. The enzyme has a pI value of 4.6 and is composed of two subunits each with a molecular weight of 23kDa. It exhibits different catalytic activities toward the substrates 1-chloro-2,4-dinitrobenzene, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ethacrynic acid, and p-nitrophenyl acetate and, fascinatingly, shows high activity toward CDNB. The amino acid composition of adGST was determined and found to be very similar to the Sloane squid GSTs. N-terminal analysis of the first 15 residues of adGST revealed that it has 73% sequence identity with the pig roundworm GSTs. The adGST shows characteristics similar to those of class sigma GSTs, as was indicated by its substrate specificity, N-terminal amino acid sequence, and amino acid composition. |
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Authors:
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Hai ling Yang; Li jia Nie; Sheng geng Zhu; Xian wan Zhou |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 403 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-07-25 Completed Date: 2002-08-22 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 202-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Chromatography, High Pressure Liquid Circular Dichroism Dinitrochlorobenzene / metabolism Glutathione Transferase / chemistry, isolation & purification*, metabolism* Intestines / enzymology Liver / enzymology Molecular Sequence Data Mollusca / enzymology* Peptide Fragments / chemistry Sequence Analysis, Protein Sequence Homology, Amino Acid Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Peptide Fragments; 97-00-7/Dinitrochlorobenzene; EC 2.5.1.18/Glutathione Transferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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