Document Detail


Purification and characterization of a novel glutathione S-transferase from Asaphis dichotoma.
MedLine Citation:
PMID:  12139969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An isoenzyme of glutathione S-transferase (adGST) was purified from liver intestine of the seashell (Asaphis dichotoma) by GST-Sepharose 4B affinity chromatography followed by reverse-phase HPLC. The enzyme has a pI value of 4.6 and is composed of two subunits each with a molecular weight of 23kDa. It exhibits different catalytic activities toward the substrates 1-chloro-2,4-dinitrobenzene, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ethacrynic acid, and p-nitrophenyl acetate and, fascinatingly, shows high activity toward CDNB. The amino acid composition of adGST was determined and found to be very similar to the Sloane squid GSTs. N-terminal analysis of the first 15 residues of adGST revealed that it has 73% sequence identity with the pig roundworm GSTs. The adGST shows characteristics similar to those of class sigma GSTs, as was indicated by its substrate specificity, N-terminal amino acid sequence, and amino acid composition.
Authors:
Hai ling Yang; Li jia Nie; Sheng geng Zhu; Xian wan Zhou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  403     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-25     Completed Date:  2002-08-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  202-8     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid
Circular Dichroism
Dinitrochlorobenzene / metabolism
Glutathione Transferase / chemistry,  isolation & purification*,  metabolism*
Intestines / enzymology
Liver / enzymology
Molecular Sequence Data
Mollusca / enzymology*
Peptide Fragments / chemistry
Sequence Analysis, Protein
Sequence Homology, Amino Acid
Substrate Specificity
Chemical
Reg. No./Substance:
0/Peptide Fragments; 97-00-7/Dinitrochlorobenzene; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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