Document Detail


Purification and characterization of a novel cysteine synthase isozyme from spinach hydrated seeds.
MedLine Citation:
PMID:  9571779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel type of cysteine synthase (CSase, EC 4.2.99.8) isozyme, designated as CSase 1', was purified to homogeneity from hydrated spinach seeds. The enzyme had a molecular weight of 68,000 and consisted of two identical subunits of M(r), 34,000. The apparent K(m) for O-acetyl-L-serine was 8.33 mM and that for sulfide was 0.66 mM. The activity of CSase 1' was maintained when it was treated at 60 degrees C for 1 min. This novel enzyme was similar to CSases 1, 2, and 3 already purified from spinach leaves, in results of double immunodiffusion, molecular weight, subunit composition, K(m) values for O-acetyl-L-serine and sulfide, and heat stability. On the other hand, N-terminal amino acid sequence, effects of immunotitration, pH optimum, and effects of hydroxylamine on purified CSase 1' were different from those of the other CSases. Furthermore, it was found that CSases 2S and 3S isolated from hydrated spinach seeds were identical with the CSases 2 and 3 reported previously. It was also disclosed that CSases 1, 2, and 3 were localized in chloroplasts, cytosol, and mitochondria, respectively.
Authors:
T Yamaguchi; X Zhu; M Masada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  62     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-05-27     Completed Date:  1998-05-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  501-7     Citation Subset:  B    
Affiliation:
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cysteine Synthase / chemistry,  isolation & purification*,  metabolism*
Enzyme Inhibitors / pharmacology
Enzyme Stability
Hot Temperature
Hydrogen-Ion Concentration
Hydroxylamine / pharmacology
Isoenzymes / chemistry,  isolation & purification*,  metabolism*
Kinetics
Molecular Sequence Data
Molecular Weight
Plant Leaves / enzymology
Plant Proteins / chemistry,  isolation & purification*,  metabolism*
Seeds / enzymology*
Sequence Homology, Amino Acid
Solubility
Spinacia oleracea / enzymology*
Water / chemistry
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Isoenzymes; 0/Plant Proteins; 7732-18-5/Water; 7803-49-8/Hydroxylamine; EC 2.5.1.47/Cysteine Synthase

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