| Purification and characterization of a newly identified growth factor specific for epithelial cells. | |
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MedLine Citation:
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PMID: 2915979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A growth factor specific for epithelial cells was identified in conditioned medium of a human embryonic lung fibroblast cell line. The factor, provisionally termed keratinocyte growth factor (KGF) because of its predominant activity on this cell type, was purified to homogeneity by a combination of ultrafiltration, heparin-Sepharose affinity chromatography, and hydrophobic chromatography on a C4 reversed-phase HPLC column. KGF was both acid and heat labile and consisted of a single polypeptide chain of approximately 28 kDa. Purified KGF was a potent mitogen for epithelial cells, capable of stimulating DNA synthesis in quiescent BALB/MK epidermal keratinocytes by greater than 500-fold with activity detectable at 0.1 nM and maximal at 1.0 nM. Lack of mitogenic activity on either fibroblasts or endothelial cells indicated that KGF possessed a target cell specificity distinct from any previously characterized growth factor. Microsequencing revealed an amino-terminal sequence containing no significant homology to any known protein. The release of this growth factor by human embryonic fibroblasts raises the possibility that KGF may play a role in mesenchymal stimulation of normal epithelial cell proliferation. |
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Authors:
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J S Rubin; H Osada; P W Finch; W G Taylor; S Rudikoff; S A Aaronson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 86 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1989 Feb |
Date Detail:
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Created Date: 1989-03-16 Completed Date: 1989-03-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 802-6 Citation Subset: IM |
Affiliation:
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Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cell Line Cells, Cultured Chromatography, Affinity Chromatography, High Pressure Liquid Epidermis / drug effects* Growth Substances / isolation & purification*, pharmacology Humans Molecular Weight Ultrafiltration |
| Chemical | |
Reg. No./Substance:
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0/Growth Substances |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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