| Purification and characterization of a neutrophil chemotactic factor from Dirofilaria immitis. | |
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MedLine Citation:
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PMID: 3525795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A neutrophil chemotactic factor (NCF-Di) was purified from a crude extract of Dirofilaria immitis adult worm by a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S-200. NCF-Di showed a single protein band by both polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS) PAGE. The molecular weight of NCF-Di was estimated to be 17,000 by gel filtration on Sephadex G-150, and 14,000 by SDS-PAGE. NCF-Di was an acidic protein with isoelectric point of 4.5. NCF-Di was absorbed neither to lentil lectin-Sepharose nor to concanavalin A-Sepharose. The chemotactic activity of NCF-Di was heat labile (56 C, 1 hr), but was resistant to periodate oxidation. These results suggest that NCF-Di is a simple peptide which has few or no sugar chains. These physicochemical properties of NCF-Di were compared to previously reported parasite-derived chemoattractants or purified allergen of D. immitis. |
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Authors:
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Y Horii; K Fujita; M Owhashi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of parasitology Volume: 72 ISSN: 0022-3395 ISO Abbreviation: J. Parasitol. Publication Date: 1986 Apr |
Date Detail:
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Created Date: 1986-09-17 Completed Date: 1986-09-17 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7803124 Medline TA: J Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 315-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemistry, Physical Chemotactic Factors / isolation & purification* Chemotaxis, Leukocyte Chromatography, Gel Chromatography, Ion Exchange Dirofilaria immitis / analysis*, parasitology Dogs Electrophoresis, Polyacrylamide Gel Filarioidea / analysis* Interleukin-8 Neutrophils / parasitology, physiology* Physicochemical Phenomena |
| Chemical | |
Reg. No./Substance:
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0/Chemotactic Factors; 0/Interleukin-8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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