Document Detail


Purification and characterization of α-neoagarooligosaccharide hydrolase from Cellvibrio sp. OA-2007.
MedLine Citation:
PMID:  24100625     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
α-Neoagarooligosaccharide (α-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular weights of α-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 kDa and 93 kDa, respectively, and the optimal temperature and pH for the enzymes's activity were 32 ºC and 7.0-7.2. α-NAOS hydrolase lost 43% of its original activity when incubated at 35 ºC for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose and neoagarohexaose to galactose, agarotriose and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly, however, did not degrade agarose.
Authors:
Osamu Ariga; Naoki Okamoto; Naomi Harimoto; Kiyohiko Nakasaki
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-10-4
Journal Detail:
Title:  Journal of microbiology and biotechnology     Volume:  -     ISSN:  1738-8872     ISO Abbreviation:  J. Microbiol. Biotechnol.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431852     Medline TA:  J Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
School of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Kami City, 782-8502, Kochi, Japan.
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