| Purification and characterization of mycoferritin from Aspergillus parasiticus (255). | |
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MedLine Citation:
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PMID: 15837384 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As intracellular iron storage molecules, only hydroxymate type siderophores have been reported in ascomycetes and basidiomycetes. This is the first report documenting the presence of mycoferritin in ascomycetes. The fungus, Aspergillus parasiticus (255), is capable of producing mycoferritin only upon induction with iron in yeast extract sucrose (YES) medium. The same has been purified from Aspergillus sps by application of conventional biochemical techniques. The molecular mass, yield, iron and carbohydrate contents of the HPLC purified protein were 460kDa, 0.012mg/g of wet mycelia, 1.6% and 6.0%, respectively. The iron content was much lower than Mortierella alpina mycoferritin (17%). Native PAGE revealed the presence of trimeric and monomeric forms of ferritin. Subunit analysis by SDS-PAGE showed a single protein subunit of approximately 20kDa suggesting structural simplicity of the apoferritin shell. Variation in amino acid composition was noted upon comparison with ferritins of other species. Interestingly, no phenylalanine could be detected in the mycoferritin of Aspergillus sps. The acidic amino acid content was 1.5-1.6 fold higher than mammalian and fish ferritins. The spectral characteristics (UV/VIS and fluorescence) of mycoferritin were akin to equine spleen ferritin. However, circular dichroic spectra revealed a lower degree of helicity. |
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Authors:
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J Shashidhar; R B Sashidhar; Vijay Deshpande |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS microbiology letters Volume: 245 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-19 Completed Date: 2005-09-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 287-93 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University College of Science, Osmania University, Hyderabad 500 007, Andhra Pradesh, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Amino Acids, Acidic / analysis Aspergillus / chemistry* Carbohydrates / analysis Circular Dichroism Electrophoresis, Polyacrylamide Gel Ferritins / chemistry*, isolation & purification* Iron / analysis Molecular Weight Phenylalanine / analysis Protein Structure, Secondary Protein Subunits / chemistry, isolation & purification Spectrum Analysis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Amino Acids, Acidic; 0/Carbohydrates; 0/Protein Subunits; 63-91-2/Phenylalanine; 7439-89-6/Iron; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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