Document Detail


Purification and characterization of mycoferritin from Aspergillus parasiticus (255).
MedLine Citation:
PMID:  15837384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As intracellular iron storage molecules, only hydroxymate type siderophores have been reported in ascomycetes and basidiomycetes. This is the first report documenting the presence of mycoferritin in ascomycetes. The fungus, Aspergillus parasiticus (255), is capable of producing mycoferritin only upon induction with iron in yeast extract sucrose (YES) medium. The same has been purified from Aspergillus sps by application of conventional biochemical techniques. The molecular mass, yield, iron and carbohydrate contents of the HPLC purified protein were 460kDa, 0.012mg/g of wet mycelia, 1.6% and 6.0%, respectively. The iron content was much lower than Mortierella alpina mycoferritin (17%). Native PAGE revealed the presence of trimeric and monomeric forms of ferritin. Subunit analysis by SDS-PAGE showed a single protein subunit of approximately 20kDa suggesting structural simplicity of the apoferritin shell. Variation in amino acid composition was noted upon comparison with ferritins of other species. Interestingly, no phenylalanine could be detected in the mycoferritin of Aspergillus sps. The acidic amino acid content was 1.5-1.6 fold higher than mammalian and fish ferritins. The spectral characteristics (UV/VIS and fluorescence) of mycoferritin were akin to equine spleen ferritin. However, circular dichroic spectra revealed a lower degree of helicity.
Authors:
J Shashidhar; R B Sashidhar; Vijay Deshpande
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  245     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-19     Completed Date:  2005-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  287-93     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University College of Science, Osmania University, Hyderabad 500 007, Andhra Pradesh, India.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Amino Acids, Acidic / analysis
Aspergillus / chemistry*
Carbohydrates / analysis
Circular Dichroism
Electrophoresis, Polyacrylamide Gel
Ferritins / chemistry*,  isolation & purification*
Iron / analysis
Molecular Weight
Phenylalanine / analysis
Protein Structure, Secondary
Protein Subunits / chemistry,  isolation & purification
Spectrum Analysis
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Amino Acids, Acidic; 0/Carbohydrates; 0/Protein Subunits; 63-91-2/Phenylalanine; 7439-89-6/Iron; 9007-73-2/Ferritins

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