Document Detail


Purification and characterization of the major glutathione transferase from adult toad (Bufo bufo) liver.
MedLine Citation:
PMID:  8424786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Five forms of glutathione transferase (GST) were resolved from the cytosol of adult common toad (Bufo bufo) liver by GSH-affinity chromatography followed by isoelectric focusing. The major enzyme (GST-7.64; 55% of total activity bound to the column) has a pI value of 7.64, is composed of two subunits each with a molecular mass of 23 kDa, and has the N-terminal amino acid residue blocked. GST-7.64 has also been characterized with respect to amino acid composition, substrate specificity, inhibition characteristics, c.d. spectra and immunological reactivity. The N-terminal sequence of some peptides obtained after tryptic digestion has also been determined. All together the results obtained suggest that the major toad liver GST is distinct from any known GST, including microbial, plant and mammalian GSTs.
Authors:
A Aceto; B Dragani; T Bucciarelli; P Sacchetta; F Martini; S Angelucci; F Amicarelli; M Miranda; C Di Ilio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  289 ( Pt 2)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-02-23     Completed Date:  1993-02-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  417-22     Citation Subset:  IM    
Affiliation:
Istituto di Scienze Biochimiche, Facoltà' di Medicina, Università G. D'Annunzio, Chieti, Italy.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Animals
Bufo bufo
Chromatography, High Pressure Liquid
Circular Dichroism
Cytosol / enzymology
Electrophoresis, Polyacrylamide Gel
Glutathione Transferase / chemistry,  isolation & purification*,  metabolism*
Isoelectric Focusing
Isoenzymes / chemistry,  isolation & purification*,  metabolism*
Kinetics
Liver / enzymology*
Macromolecular Substances
Molecular Sequence Data
Molecular Weight
Substrate Specificity
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Isoenzymes; 0/Macromolecular Substances; EC 2.5.1.18/Glutathione Transferase
Comments/Corrections

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