Document Detail

Purification and characterization of a lactonase from Erwinia cypripedii 314B that hydrolyzes ( S)-5-oxo-2-tetrahydrofurancarboxylic acid.
MedLine Citation:
PMID:  14576976     Owner:  NLM     Status:  MEDLINE    
A bacterium, strain 314B, able to assimilate ( S)-5-oxo-2-tetrahydrofurancarboxylic acid was isolated from soil and identified as Erwinia cypripedii. A lactonase hydrolyzing ( S)-5-oxo-2-tetrahydrofurancarboxylic acid to l-alpha-hydroxyglutaric acid was purified 63-fold with 2% recovery from crude extracts of this bacterium to homogeneity as judged by SDS-PAGE. The molecular masses estimated by SDS-PAGE and gel filtration were 41 kDa and 79 kDa, respectively. The maximum activity was observed at pH 6.5-7.5 and 35-45 degrees C. The enzyme showed lower activity toward dl-2-oxotetrahydrofuran-4,5-dicarboxylic acid, but did not act on ( R)-5-oxo-2-tetrahydrofurancarboxylic acid and other natural and synthetic lactones tested.
Kazuya Mochizuki
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Publication Detail:
Type:  Journal Article     Date:  2003-10-24
Journal Detail:
Title:  Archives of microbiology     Volume:  180     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-05     Completed Date:  2004-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  490-3     Citation Subset:  IM    
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1-1 Higashi, 305-8566 Tsukuba, Ibaraki, Japan.
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MeSH Terms
Amino Acid Sequence
Ammonium Sulfate
Cations, Divalent / metabolism
Chromatography / methods
Electrophoresis, Polyacrylamide Gel / methods
Enzyme Inhibitors / pharmacology
Enzyme Stability
Erwinia / enzymology*
Fungal Proteins / antagonists & inhibitors,  chemistry,  isolation & purification*,  metabolism*
Furans / metabolism*
Hydrogen-Ion Concentration
Hydrolases / antagonists & inhibitors,  chemistry,  isolation & purification*,  metabolism*
Metals / metabolism
Molecular Sequence Data
Molecular Weight
Substrate Specificity
Reg. No./Substance:
0/5-oxo-2-tetrahydrofurancarboxylic acid; 0/Cations, Divalent; 0/Enzyme Inhibitors; 0/Fungal Proteins; 0/Furans; 0/Metals; 0/Polymers; 1306-06-5/Durapatite; 7783-20-2/Ammonium Sulfate; 90327-94-9/butyl-Toyopearl 650; EC 3.-/Hydrolases; EC 3.-/Zhd101 protein, Bionectria ochroleuca

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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