Document Detail

Purification and characterization of human phosphatidylserine synthases 1 and 2.
MedLine Citation:
PMID:  19014349     Owner:  NLM     Status:  MEDLINE    
PS (phosphatidylserine) in mammalian cells is synthesized by two distinct base-exchange enzymes, PSS1 (PS synthase 1) and PSS2, which are responsible for the conversion of PC (phosphatidylcholine) and PE (phosphatidylethanolamine) respectively into PS in intact cells. The PS synthesis in cultured mammalian cells is inhibited by exogenous PS, and this feedback control occurs through inhibition of PSSs by PS. In the present study, we purified epitope-tagged forms of human PSS1 and PSS2. The purified PSS2 was shown to catalyse the conversion of PE, but not PC, into PS, this being consistent with the substrate specificity observed in intact cells. On the other hand, the purified PSS1 was shown to catalyse the conversion of both PC and PE into PS, although PSS1 in intact cells had been shown not to contribute to the conversion of PE into PS to a significant extent. Furthermore, we found that the purified PSS2, but not the purified PSS1, was inhibited on the addition of PS to the enzyme assay mixture, raising the possibility that there was some difference between the mechanisms of the inhibitory actions of PS towards PSS1 and PSS2.
Shiho Tomohiro; Ayako Kawaguti; Yukiyo Kawabe; Sakae Kitada; Osamu Kuge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  418     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-06     Completed Date:  2009-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  421-9     Citation Subset:  IM    
Department of Chemistry, Faculty of Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
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MeSH Terms
Cloning, Molecular
DNA, Complementary / isolation & purification
Enzyme Activation / drug effects
Hela Cells
Hemagglutinins / chemistry
Nitrogenous Group Transferases / chemistry,  genetics*,  isolation & purification*,  metabolism
Peptides / chemistry
Phosphatidylethanolamines / pharmacology
Phosphatidylserines / pharmacology
Recombinant Proteins / chemistry,  isolation & purification
Reg. No./Substance:
0/DNA, Complementary; 0/Hemagglutinins; 0/Peptides; 0/Phosphatidylethanolamines; 0/Phosphatidylserines; 0/Recombinant Proteins; 39382-08-6/phosphatidylethanolamine; 98849-88-8/FLAG peptide; EC 2.6.-/Nitrogenous Group Transferases; EC 2.6.99.-/Ptdss2 protein, mouse; EC 2.7.8.-/phospholipid serine base exchange enzyme

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