| Purification and characterization of heparin-binding endothelial cell growth factors. | |
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MedLine Citation:
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PMID: 3003088 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thirteen endothelial cell growth factors have been purified to homogeneity by heparin affinity and reversed-phase high performance liquid chromatography, and their chromatographic and electrophoretic properties were compared. The amino acid compositions of 10 of these mitogens have also been determined. The results indicate that these heparin-binding growth factors (HBGFs) can be subdivided into two classes. Class 1 HBGFs are anionic mitogens of molecular weight 15,000-17,000 found in high levels in neural tissue and include acidic brain fibroblast growth factor and retina-derived growth factor. Class 2 HBGFs are cationic mitogens of molecular weight 18,000-20,000 found in a variety of normal tissues and are typified by pituitary fibroblast growth factor and cartilage-derived growth factor. Typical class 2 HBGFs have also been isolated from a rat chondrosarcoma, a human melanoma, and a human hepatoma, suggesting that tumors do not make a structurally distinct HBGF class. These results provide a sound basis for the evaluation of the HBGFs purified from a variety of tissues and species and for the delineation of their normal and pathological functions in vivo. |
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Authors:
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R Lobb; J Sasse; R Sullivan; Y Shing; P D'Amore; J Jacobs; M Klagsbrun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 261 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1986 Feb |
Date Detail:
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Created Date: 1986-03-12 Completed Date: 1986-03-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1924-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Brain Chemistry Carcinoma, Hepatocellular / analysis Cattle Cell Line Chickens Chondrosarcoma / analysis Chromatography, Affinity Chromatography, High Pressure Liquid Endothelial Growth Factors Fibrosarcoma / analysis Growth Substances / classification, isolation & purification*, metabolism Heparin / metabolism* Humans Hypothalamus / analysis Isoelectric Point Liver Neoplasms / analysis Melanoma / analysis Molecular Weight Neoplasm Proteins / isolation & purification, metabolism Rats Retina / analysis |
| Grant Support | |
ID/Acronym/Agency:
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EY05985/EY/NEI NIH HHS; R01 CA37392/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Endothelial Growth Factors; 0/Growth Substances; 0/Neoplasm Proteins; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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