Document Detail

Purification and characterization of ferredoxin-sulfite reductase from turnip (Brassica rapa) leaves and comparison of properties with ferredoxin-sulfite reductase from turnip roots.
MedLine Citation:
PMID:  9339549     Owner:  NLM     Status:  MEDLINE    
Ferredoxin-sulfite reductase (Fd-SiR) [hydrogen-sulfide: ferredoxin oxidoreductase, EC] from turnip leaves (SiR-L) has been purified to homogeneity and its enzymatic properties compared with that from turnip roots (SiR-R). Each enzyme had a molecular mass of 64.5 +/- 0.5 kDa by SDS-PAGE and an isoelectric point of 5.15 +/- 0.05. Although each had a pH optimum around 7.8 with the same effects of inhibitors, SiR-L had higher heat stability at 60 degrees C than SiR-R. Moreover, SiR-R had a lower K(m) and a higher specificity constant (kcat/K(m)) for turnip leaf ferredoxin than SiR-L. The N-terminal amino acid sequence of SiR-L was different from that of SiR-R. The results of amino acid analysis and peptide mapping suggested that SiR-L and SiR-R have different primary structures.
S Takahashi; W C Yip; G Tamura
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  61     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-02-05     Completed Date:  1998-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1486-90     Citation Subset:  B    
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, Japan.
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Arabidopsis Proteins*
Brassica / enzymology*
Electrophoresis, Polyacrylamide Gel
Isoelectric Focusing
Molecular Sequence Data
Molecular Weight
Oxidoreductases Acting on Sulfur Group Donors / chemistry,  isolation & purification*
Peptide Mapping
Plant Leaves / enzymology*
Plant Proteins / analysis,  isolation & purification
Plant Roots / enzymology*
Sulfite Reductase (Ferredoxin)
Sulfites / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Arabidopsis Proteins; 0/Plant Proteins; 0/Sulfites; EC 1.8.-/Oxidoreductases Acting on Sulfur Group Donors; EC protein, Arabidopsis; EC Reductase (Ferredoxin)

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