Document Detail


Purification and characterization of ferredoxin-sulfite reductase from turnip (Brassica rapa) leaves and comparison of properties with ferredoxin-sulfite reductase from turnip roots.
MedLine Citation:
PMID:  9339549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ferredoxin-sulfite reductase (Fd-SiR) [hydrogen-sulfide: ferredoxin oxidoreductase, EC 1.8.7.1] from turnip leaves (SiR-L) has been purified to homogeneity and its enzymatic properties compared with that from turnip roots (SiR-R). Each enzyme had a molecular mass of 64.5 +/- 0.5 kDa by SDS-PAGE and an isoelectric point of 5.15 +/- 0.05. Although each had a pH optimum around 7.8 with the same effects of inhibitors, SiR-L had higher heat stability at 60 degrees C than SiR-R. Moreover, SiR-R had a lower K(m) and a higher specificity constant (kcat/K(m)) for turnip leaf ferredoxin than SiR-L. The N-terminal amino acid sequence of SiR-L was different from that of SiR-R. The results of amino acid analysis and peptide mapping suggested that SiR-L and SiR-R have different primary structures.
Authors:
S Takahashi; W C Yip; G Tamura
Related Documents :
20047649 - Amino acid "little big bang": representing amino acid substitution matrices as dot prod...
1567369 - Glutathione transferase isoenzymes from bufo bufo embryos at an early developmental stage.
24741159 - Cloud-point extraction and reversed-phase high performance liquid chromatography for an...
25049789 - Antioxidant activities of achyranthes japonica nakai extract and its application to the...
24637609 - Versatile c3-symmetric scaffolds and their use for covalent stabilization of the foldon...
24271629 - A saturable site responsible for polar transport of indole-3-acetic acid in sections of...
9820969 - Humic acid capillary zone electrophoresis adsorption on capillary walls, separation in ...
10794609 - Spanish sparkling wines (cavas) as inhibitors of in vitro human low-density lipoprotein...
3032679 - A commentary on the inhibition by retinoids of leukotriene b4 production in leukocytes.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  61     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-02-05     Completed Date:  1998-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1486-90     Citation Subset:  B    
Affiliation:
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Arabidopsis Proteins*
Brassica / enzymology*
Electrophoresis, Polyacrylamide Gel
Isoelectric Focusing
Kinetics
Molecular Sequence Data
Molecular Weight
Oxidoreductases Acting on Sulfur Group Donors / chemistry,  isolation & purification*
Peptide Mapping
Plant Leaves / enzymology*
Plant Proteins / analysis,  isolation & purification
Plant Roots / enzymology*
Sulfite Reductase (Ferredoxin)
Sulfites / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Arabidopsis Proteins; 0/Plant Proteins; 0/Sulfites; EC 1.8.-/Oxidoreductases Acting on Sulfur Group Donors; EC 1.8.7.1/SIR protein, Arabidopsis; EC 1.8.7.1/Sulfite Reductase (Ferredoxin)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The effects of corn peptide ingestion on facilitating alcohol metabolism in healthy men.
Next Document:  Subcellular location of polyphenol oxidase in apples.