| Purification and characterization of equine relaxin. | |
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MedLine Citation:
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PMID: 3732157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been previously determined that the equine placenta is the sole significant source of relaxin during pregnancy and that relaxin immunoactivity is also present in term placentas. Therefore, placentas obtained at the time of foaling were selected for starting material for purification of equine relaxin. Frozen whole placentas were ground and then extracted with 0.5 N HCl-85% acetone. Relaxin was precipitated by raising the acetone concentration to 97%. Equine relaxin was further purified by stepwise elution ion exchange, gel filtration, and gradient elution ion exchange chromatographies and trichloroacetic acid precipitation. Equine relaxin purified in this manner was shown to be heterogeneous with one major form (R-1) and several minor forms (two of which are identified as R-2 and R-3). About 1.5 mg R-1 were obtained per kg placenta. Purity was assessed by slab and disc gel electrophoresis and dansyl end group analysis. R-1 had a potency of 28 U/mg, as measured in the mouse inter-pubic ligament bioassay and displayed a dose-response curve parallel with porcine relaxin. Amino acid analysis indicated the presence of tyrosine, histidine, and proline, amino acids absent in porcine relaxin. Dansyl end group analysis indicated the blockage of N-terminal groups on R-1 and the presence of Lys on R-3. A lower molecular weight was indicated by the electrophoretic migration of relaxin reduced with 2-mercaptoethanol suggesting that equine relaxin consists of two chains. |
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Authors:
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D R Stewart; H Papkoff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 119 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1986 Sep |
Date Detail:
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Created Date: 1986-09-19 Completed Date: 1986-09-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1093-9 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adnexa Uteri
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drug effects Amino Acids / analysis Animals Biological Assay Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Female Horses Mice Placenta / analysis Pregnancy Relaxin / isolation & purification* |
| Grant Support | |
ID/Acronym/Agency:
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HD-05722/HD/NICHD NIH HHS; HD-06386/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 9002-69-1/Relaxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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