| Purification, characterization and cytotoxicity of malanin, a novel plant toxin from the seeds of Malania oleifera. | |
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MedLine Citation:
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PMID: 19341757 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Malanin, a novel plant toxin with a molecular weight of 61,875 Da and an isoelectric point of 5.5, was isolated from Malania oleifera seeds by homogenization, ammonium sulfate precipitation and hydrophobic interaction chromatography (HIC). It is a glycoprotein with two chains, chain-A and chain-B, which are crosslinked by one or more disulfide bonds. The N-terminal amino-acid sequences of malanin are DETXTDEEFN (X was commonly C) in chain-B, and DYPKLTFTTS in chain-A. Malanin exhibited highly cytotoxic activities against cancer cell lines (HeLa, PC-12, MCF-7, K562) and non-cancer cell lines (Vero and MDCK), producing IC(50) values of 0.15+/-0.08, 7.71+/-0.24, 11.20+/-0.02, 15.80+/-0.09, 2.79+/-0.05 and 3.92+/-0.01 nM, respectively. It significantly inhibited the growth of HeLa cells through cell-cycle arrest at S phase and induced an apoptotic response. LD(50) values were determined in ICR mice, which were found to be 26.22 microg/kg and 43.11 mg/kg by i.p. and i.g. respectively. Thus, malanin is amongst the most potent toxin of plant origin. |
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Authors:
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Yan Yuan; Xiaochang Dai; Debin Wang; Xianghui Zeng |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-01 |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 54 ISSN: 1879-3150 ISO Abbreviation: Toxicon Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-06-01 Completed Date: 2009-09-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: England |
Other Details:
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Languages: eng Pagination: 121-7 Citation Subset: IM |
Affiliation:
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Department of Life Science and Technology, Kunming University, Kunming 650031, China. yuanyanfy@yahoo.com.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis Animals Carbohydrates / analysis Cell Line, Tumor Female Glycoproteins / analysis, chemistry*, toxicity* Hela Cells Humans Isoelectric Focusing Male Mass Spectrometry Mice Mice, Inbred ICR Molecular Sequence Data Molecular Weight Olacaceae / chemistry* Seeds / chemistry Toxins, Biological / chemistry*, toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Glycoproteins; 0/Toxins, Biological; 0/malanin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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