Document Detail

Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia.
MedLine Citation:
PMID:  2598320     Owner:  NLM     Status:  MEDLINE    
A novel cytolytic factor, dolabellanin P, was purified to apparent homogeneity from the purple fluid of the sea hare, Dolabella auricularia. Purified dolabellanin P is a single polypeptide of 60 kDa. The amino acid composition and the N-terminus of the factor were also determined. This factor nonspecifically lysed all the cells tested at 50-200 ng protein/ml. Dolabellanin P caused complete cytolysis within 2 h. The factor is distinct from antineoplastic glycoproteins previously isolated from eggs (aplysianin E) or albumen gland (aplysianin A) of Aplysia kurodai in terms of certain cytolytic properties. These results suggest that dolabellanin P, found in the sea hare, a marine invertebrate, is a new cytolytic factor.
M Yamazaki; S Tansho; J Kisugi; K Muramoto; H Kamiya
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemical & pharmaceutical bulletin     Volume:  37     ISSN:  0009-2363     ISO Abbreviation:  Chem. Pharm. Bull.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1990-02-06     Completed Date:  1990-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0377775     Medline TA:  Chem Pharm Bull (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  2179-82     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Cell Survival / drug effects
Glycoproteins / isolation & purification*,  pharmacology
Mice, Inbred C3H
Molecular Sequence Data
Mollusca / analysis*
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Glycoproteins; 123758-92-9/dolabellanin P protein, Dolabella auricularia

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