Document Detail

Purification, characterization and cytokine release function of a novel Arg-49 phospholipase A(2) from the venom of Protobothrops mucrosquamatus.
MedLine Citation:
PMID:  16793192     Owner:  NLM     Status:  MEDLINE    
Group IIA phospholipase A(2) (PLA(2)) are major components in Viperidae/Crotalidae venom. In the present study, a novel PLA(2) named promutoxin with Arg at the site 49 has been purified from the venom of Protobothrops mucrosquamatus by chromatography. It consists of 122 amino acid residues with a molecular mass of 13,656 Da assessed by MALDI-TOF. It has the structural features of snake venom group IIA PLA(2)s, but has no PLA(2) enzymatic activity. Promutoxin shows higher amino acid sequence identity to the K49 PLA(2)s (72-95%) than to D49 PLA(2)s (52-58%). Promutoxin exhibits potent myotoxicity in the animal model with as little as 1 microg of promutoxin causing myonecrosis and myoedema in the gastrocnemius muscle of mice. Promutoxin is also able to stimulate the release of IL-12, TNFalpha, IL-6 and IL-1beta from human monocytes, and induce IL-2, TNFalpha and IL-6 release from T cells, indicating that this snake venom group IIA PLA(2) is actively involved in the inflammatory process in man caused by snake venom poisoning.
Ji-Fu Wei; Tao Li; Xiao-Long Wei; Qian-Yun Sun; Fu-Mei Yang; Qiu-Yu Chen; Wan-Yu Wang; Yu-Liang Xiong; Shao-Heng He
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-14
Journal Detail:
Title:  Biochimie     Volume:  88     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-11-07     Completed Date:  2007-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  1331-42     Citation Subset:  IM    
Allergy and Inflammation Research Institute, The Shantou University Medical College, Xinling Road 11, 515031 Shantou, Guangdong, China.
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MeSH Terms
Amino Acid Sequence
Anti-Bacterial Agents / pharmacology
Base Sequence
Crotalid Venoms / enzymology*
Cytokines / metabolism*
Molecular Sequence Data
Molecular Weight
Monocytes / drug effects,  immunology
Phospholipases A / chemistry*,  isolation & purification,  pharmacology*
Rats, Wistar
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
T-Lymphocytes / drug effects,  immunology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Crotalid Venoms; 0/Cytokines; 74-79-3/Arginine; EC 3.1.1.-/Phospholipases A

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