Document Detail


Purification and characterization of a corrinoid compound from Chlorella tablets as an algal health food.
MedLine Citation:
PMID:  12166996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vitamin B(12) content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 microg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 microg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy.
Authors:
Hiromi Kittaka-Katsura; Tomoyuki Fujita; Fumio Watanabe; Yoshihisa Nakano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  50     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-08     Completed Date:  2002-09-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4994-7     Citation Subset:  IM    
Affiliation:
Department of Health Science, Hiroshima Prefectural Women's University, Hiroshima 734-8558, Japan.
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MeSH Terms
Descriptor/Qualifier:
Chemiluminescent Measurements
Chlorella / chemistry*
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Corrinoids
Health Food / analysis*
Magnetic Resonance Spectroscopy
Porphyrins / chemistry*,  isolation & purification*
Spectrophotometry, Ultraviolet
Vitamin B 12 / chemistry,  isolation & purification
Chemical
Reg. No./Substance:
0/Corrinoids; 0/Porphyrins; 68-19-9/Vitamin B 12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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