Document Detail

Purification and characterization of chromium-binding substances from high-chromium yeast.
MedLine Citation:
PMID:  23320760     Owner:  NLM     Status:  MEDLINE    
As a highly efficient and easily absorbable source of chromium, the identities of the chromium-binding substances in yeast remain unclear. In this study, a mild extraction procedure involving extraction with ammonia, three-gel filtration, and high-performance liquid chromatography was adopted to obtain two chromium-binding substances from high-chromium yeast. A low-molecular-weight chromium-binding substance was identified, with mass-to-charge ratios (m/z) of 769 and 712, which included glutamic acid, glycine, and cysteine in an approximate ratio of 1:1:1, as well as nicotinic acid and chromium(III). Furthermore, it significantly potentiated (by 51%) the action of insulin to stimulate the conversion of (14)C-glucose into lipid in adipocytes. A novel high-molecular-weight chromium-binding substance was also isolated: electrospray ionization tandem mass spectrometry tentatively identified it as HUB1 target protein-1, glyceraldehyde-3-phosphate dehydrogenase, or ribosomal protein L2A(L5A)(rp8)(YL6). This is the first report of a high-molecular-weight chromium-binding substance in yeast and merits further studies.
Lu Liu; Jia-ping Lv; Hankie Uluko
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-02-04
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  61     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-13     Completed Date:  2013-11-18     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1279-87     Citation Subset:  IM    
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MeSH Terms
Carrier Proteins / chemistry*,  isolation & purification,  metabolism
Chromium / analysis,  metabolism*
Molecular Weight
Saccharomyces cerevisiae / chemistry*,  metabolism
Saccharomyces cerevisiae Proteins / chemistry*,  isolation & purification,  metabolism
Spectrometry, Mass, Electrospray Ionization
Reg. No./Substance:
0/Carrier Proteins; 0/Saccharomyces cerevisiae Proteins; 0R0008Q3JB/Chromium
Comment In:
J Agric Food Chem. 2013 Sep 25;61(38):9282-4   [PMID:  23977823 ]
J Agric Food Chem. 2013 Sep 25;61(38):9280-1   [PMID:  23978315 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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