| Purification, characterization and cellular localization of 5'-nucleotidase from Torpedo electric organ. | |
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MedLine Citation:
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PMID: 2822031 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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5'-Nucleotidase was isolated from the electric organ of the electric ray Torpedo marmorata after solubilization in Triton X-100 and deoxycholate by affinity chromatography on concanavalin A-Sepharose and AMP-Sepharose. The purified enzyme has a Km for AMP of 38 microM, with a maximal velocity of 31 units/mg of protein. Of the purine and pyrimidine mononucleotides, AMP is hydrolysed most effectively. beta-Glycerophosphate, phosphoenolpyruvate and p-nitrophenyl phosphate are not substrates for the enzyme. Adenosine 5'-[alpha, beta-methylene]diphosphate, ADP and ATP are competitive inhibitors in this order of potency. Concanavalin A inhibits enzyme activity in a non-competitive manner. Whereas Mg2+, Ca2+ and Sr2+ activate enzyme activity in the millimolar range, Hg2+, and in particular Pb2+ and Zn2+, inhibit enzyme activity. On SDS/polyacrylamide-gel electrophoresis the enzyme has an apparent Mr of 62000, whereas that of the native deoxycholate-enzyme complex is 131000. An antiserum raised against the native enzyme inhibits enzyme activity. Inhibition studies suggest the presence of tissue-specific variants of the enzyme. By immunohistochemical analysis the enzyme can be localized to the ramifications of nerve terminals in the electric organ. |
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Authors:
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E J Grondal; H Zimmermann |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 245 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1987 Aug |
Date Detail:
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Created Date: 1987-11-18 Completed Date: 1987-11-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 805-10 Citation Subset: IM |
Affiliation:
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AK Neurochemie, Zoologisches Institut der J.W. Goethe-Universitt, Frankfurt am Main, Federal Republic of Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5'-Nucleotidase Animals Cations, Divalent / pharmacology Electric Organ / enzymology* Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Histocytochemistry Immune Sera / pharmacology Nucleotidases / antagonists & inhibitors, isolation & purification, metabolism* Substrate Specificity Tissue Distribution Torpedo / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cations, Divalent; 0/Immune Sera; EC 3.1.3.-/Nucleotidases; EC 3.1.3.5/5'-Nucleotidase |
| Comments/Corrections | |
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