Document Detail


Purification and characterization of a bovine pregnancy-associated glycoprotein.
MedLine Citation:
PMID:  1908709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A 67000 Mr bovine pregnancy-associated glycoprotein (bPAG) has been isolated from fetal cotyledons and purified to homogeneity by HPLC. The purification was monitored by a double immunodiffusion test and by RIA in conjunction with an antiserum raised against a crude fraction of placenta-specific antigens. The molecular weight of bPAG was estimated to be 67000 by SDS-PAGE. The isoelectric points (pI) of the four isoforms, determined by high-resolution analytical electrofocusing in polyacrylamide gel, were 4.4, 4.6, 5.2, and 5.4. The carbohydrate content of the bPAG consisted of approximately 10.02 +/- 1.09% neutral sugar and variant amounts of sialic acid (from 0.29 +/- 0.06% in the most basic isoform to 2.1 +/- 0.31% in the most acidic isoform). A specific antiserum was raised against the purified bPAG. A specific RIA showed that the bPAG was antigenically unrelated to BSA, alphafetoprotein (AFP), and human schwangerschafts-spezifischen (pregnancy-specific) beta 1 glycoprotein (SP1). According to some characteristics (e.g. the molecular weight), the purified bPAG may correspond to a form of the pregnancy-specific protein B previously described by Sasser and colleagues (Biol Reprod 1986; 35:936-942).
Authors:
A P Zoli; J F Beckers; P Wouters-Ballman; J Closset; P Falmagne; F Ectors
Related Documents :
11821299 - Serum free beta-hcg and alpha-fetoprotein levels in ivf, icsi and frozen embryo transfe...
20653339 - Recurrent miscarriage and hcg supplementation: a review and metaanalysis.
4015199 - Routine quantification of rheumatoid factor by rate nephelometry.
17509589 - Assessment of myoelectrical signal parameters in estrogen, progesterone, and human chor...
1521649 - The impact of embryonic development and endometrial maturity on the timing of implantat...
6413269 - Effect of a partially desialylated human chorionic gonadotropin preparation (desialo hc...
8829069 - Fatal lupus pleuritis presenting in pregnancy. a case report.
18797729 - Physical activity during pregnancy and maternal-child health outcomes: a systematic lit...
7669389 - Neonatal haemochromatosis associated with down syndrome.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  45     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Affiliation:
Département d'Endocrinologie et de Reproduction Animales (Unité de Recherche IRSIA) Faculté de Médecine Vétérinaire, Bruxelles, Belgique.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Animals
Carbohydrates / analysis
Cattle
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Female
Immune Sera / immunology
Immunoblotting
Immunodiffusion
Isoelectric Focusing
Molecular Sequence Data
Molecular Weight
N-Acetylneuraminic Acid
Pregnancy
Pregnancy-Specific beta 1-Glycoproteins / immunology,  isolation & purification*,  metabolism
Radioimmunoassay
Sialic Acids / analysis
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Immune Sera; 0/Pregnancy-Specific beta 1-Glycoproteins; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Serum erythropoietic inhibitors in patients with pure red cell aplasia.
Next Document:  Age-related changes in the function of the pituitary-gonadal axis in a sterile male rat mutant (hd/h...