Document Detail

Purification and characterization of a bovine pregnancy-associated glycoprotein.
MedLine Citation:
PMID:  1908709     Owner:  NLM     Status:  MEDLINE    
A 67000 Mr bovine pregnancy-associated glycoprotein (bPAG) has been isolated from fetal cotyledons and purified to homogeneity by HPLC. The purification was monitored by a double immunodiffusion test and by RIA in conjunction with an antiserum raised against a crude fraction of placenta-specific antigens. The molecular weight of bPAG was estimated to be 67000 by SDS-PAGE. The isoelectric points (pI) of the four isoforms, determined by high-resolution analytical electrofocusing in polyacrylamide gel, were 4.4, 4.6, 5.2, and 5.4. The carbohydrate content of the bPAG consisted of approximately 10.02 +/- 1.09% neutral sugar and variant amounts of sialic acid (from 0.29 +/- 0.06% in the most basic isoform to 2.1 +/- 0.31% in the most acidic isoform). A specific antiserum was raised against the purified bPAG. A specific RIA showed that the bPAG was antigenically unrelated to BSA, alphafetoprotein (AFP), and human schwangerschafts-spezifischen (pregnancy-specific) beta 1 glycoprotein (SP1). According to some characteristics (e.g. the molecular weight), the purified bPAG may correspond to a form of the pregnancy-specific protein B previously described by Sasser and colleagues (Biol Reprod 1986; 35:936-942).
A P Zoli; J F Beckers; P Wouters-Ballman; J Closset; P Falmagne; F Ectors
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  45     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Département d'Endocrinologie et de Reproduction Animales (Unité de Recherche IRSIA) Faculté de Médecine Vétérinaire, Bruxelles, Belgique.
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Carbohydrates / analysis
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Immune Sera / immunology
Isoelectric Focusing
Molecular Sequence Data
Molecular Weight
N-Acetylneuraminic Acid
Pregnancy-Specific beta 1-Glycoproteins / immunology,  isolation & purification*,  metabolism
Sialic Acids / analysis
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Immune Sera; 0/Pregnancy-Specific beta 1-Glycoproteins; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid

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